- ￥5000
- GeneTex
- 美国
- GTX20818
- 2025年07月08日
- WB, ICC/IF, IP, Gel supershift assays
- Mouse
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human TBP.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX20818
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IP, Gel supershift assays
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
This antibody recognizes an epitope within amino acid residues 1-20 of human, mouse and rat TBP.,We do not recommend use of this product for Drosophila,Silk worm,Xenopus,Yeast.
- 抗体英文名：
TBP antibody [1TBP18]
- 抗体名：
TBP 抗体 [1TBP18]
- 规格：
100 μg
WB analysis of ecombinant human TATA Binding Protein, HeLa cell nuclear extract (A), and TBP protein fragment lysate (B) using GTX20818 TBP antibody [1TBP18].
Dilution : 2μg/ml
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文献和实验Lee LC et al., PLoS One 2014 (PMID:25549101)
Revet I et al., Cancer Lett 2010 (PMID:19815336)
Western Blot of TBP from TBP-GST bacteria
Culture bacteria 1. Grow glycerol stock A13 (TBP-GST in BL21) in 10ml LB and 10μl ampicillin (1:1000) 2. Shake @ 37℃ ON Induce bacteria 3. Adjust culture with LB until ON culture OD [@ 600nm is ~0.6-0.8 4. Induce with 40mM IPTG (made fresh
Purification of the human TATA-Binding Protein, TBP
The TATA-binding protein TBP plays a central role in eukaryotic transcription initiation (1 , 2 ). TBP binds to the promoter consensus sequence TATAAA, located approx 30 nucleotides upstream of the transcriptional start site. Once bound, TBP
Using FRET to Monitor Protein-Induced DNA Bending: The TBP-TATA Complex as a Model System
Proteins that bind to DNA can elicit changes in DNA conformation, such as bending and looping, which are important signals for later events such as transcription. TATA-binding protein (TBP) is one example of a protein that elicits
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