- ￥6300
- GeneTex
- 美国
- GTX11304
- 2025年07月11日
- WB, ICC/IF, IP
- Mouse
- Human, Mouse, Rat, Zebrafish, Bovine, Dog, Chicken, Monkey, African green monkey, Kangaroo rat, Sea Urchin, Chlamydomonas
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Sarkosyl-resistant filaments from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Zebrafish, Bovine, Dog, Chicken, Monkey, African green monkey, Kangaroo rat, Sea Urchin, Chlamydomonas
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Sea Urchin
- 目录编号：
GTX11304
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IP
- 浓度：
~2? mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Recognizes an epitope located at the C-terminal end of the α-tubulin isoform in a variety of organisms.
- 抗体英文名：
alpha Tubulin antibody [B-5-1-2]
- 抗体名：
alpha Tubulin 抗体 [B-5-1-2]
- 规格：
100 μl
WB analysis of (1) HeLa, (2) Jurkat, (3) COS7, (4) NIH-3T3, (5) PC-12, (6) RAT2, (7) CHO, (8) MDBK and (9) MDCK lysates using alpha Tubulin antibody [B-5-1-2] at 0.5 μg/ml.
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文献和实验Huang AM et al., Biochem Pharmacol 2011 (PMID:21477582)
Koening CL et al., Blood 2009 (PMID:19587376)
De Domenico I et al., Proc Natl Acad Sci U S A 2009 (PMID:19234114)
Paradkar PN et al., Mol Cell Biol 2009 (PMID:19075006)
Paradkar PN et al., Blood 2008 (PMID:18369153)
Auer TO et al., Elife 2015 (PMID:26076409)
De Domenico I et al., Cell Metab 2011 (PMID:22019085)
Immunofluorescent Localization of Tubulin
antibody to tubulin) FITC-labeled goat anti-rabbit (or secondary antibody to match the primary) Fluorescence Mounting Media Fluorescent Microscope equipped with 490 nm excitation filter and 515 nmbarrier filter Kodachrome film
Large Scale Tubulin Preparation
Tubulin is purified from bovine/porcine brain by two cycles of polymerization/depolymerization followed by removal of copurifying proteins on a phosphocellulose (PC) column. The procedure described here is for a large scale prep (10 cow brains
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
Permeabilize in TBS-0.5% TX for 10 minutes Rinse in TBS-0.1% TX (3 changes in 3-5 minutes is adequate) Block in Abdil for 10 minutes Add primary antibody diluted in Abdil for 1-1.5 hours Wash in TBS-0.1%TX (5 changes
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