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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
- 克隆性:
Monoclonal
- 标记物:
FITC
- 适应物种:
Human, Mouse, Rat, Bovine, Hamster, Pig, Baboon, Carp
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX78442
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
ICC/IF, FACS, IHC
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
This antibody recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human betaIII-tubulin.
- 抗体英文名:
beta Tubulin 3/ Tuj1 antibody [TU-20] (FITC)
- 抗体名:
beta Tubulin 3/ Tuj1 抗体 [TU-20] (FITC)
- 规格:
100 μg
ICC/IF analysis of Neuro2a cells using GTX78442 beta Tubulin 3/ Tuj1 antibody [TU-20] (FITC).
Green : Primary antibody
Blue : DAPI
Diultion : 1μg/ml
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文献和实验Jung G et al., The Korean Journal of Pathology. 2011 45()
Park JH et al., J Korean Neurosurg Soc 2012 (PMID:23115670)
Immunofluorescent Localization of Tubulin
antibody to tubulin) FITC-labeled goat anti-rabbit (or secondary antibody to match the primary) Fluorescence Mounting Media Fluorescent Microscope equipped with 490 nm excitation filter and 515 nmbarrier filter Kodachrome film
Immunofluorescent Localization of Tubulin
Rabbit anti-tubulin (or other primary antibody to tubulin) FITC-labeled goat anti-rabbit (or secondary antibody to match the primary) Fluorescence Mounting Media Fluorescent Microscope equipped with 490 nm excitation filter
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
immunofluorescence. Block in AbDil for 10'. Anti-tubulin for 20' - 30' (We use DM1alpha) Wash 4x TBST (TBST = TBS + 0.1% Triton X-100) Secondary for 20' - 30' Wash 4X TBST. Wash once with TBST + 1 ug/ml Hoechst
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