- ￥5000
- GeneTex
- 美国
- GTX27751
- 2025年07月11日
- WB, ICC/IF, IHC-P, FACS, IHC
- Mouse
- Human, Mouse, Rat, Bovine, Hamster, Pig, Baboon, Carp
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Bovine, Hamster, Pig, Baboon, Carp
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX27751
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P, FACS, IHC
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
This antibody recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human betaIII-tubulin.
- 抗体英文名：
beta Tubulin 3/ Tuj1 antibody [TU-20]
- 抗体名：
beta Tubulin 3/ Tuj1 抗体 [TU-20]
- 规格：
100 μg
ICC/IF analysis of Neuro2a cells using GTX27751 beta Tubulin 3/ Tuj1 antibody [TU-20].
Green : Primary antibody
Blue : DAPI
Diultion : 3μg/ml
ICC/IF analysis of P-19 cells stimulated to neuronal differentiation by retinoic acid using GTX27751 beta Tubulin 3/ Tuj1 antibody [TU-20] and GTX27792 beta Tubulin antibody [TU-06].
Panel A : GTX27751 (Red)
Panel B : GTX27751 (Red) and GTX27792 (Green)
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文献和实验Schaefers C et al., Stem Cells Int 2022 (PMID:36530489)
Liu KC et al., Oncotarget 2013 (PMID:24280306)
Lehmann M et al., PLoS One 2013 (PMID:23936474)
Jung G et al., The Korean Journal of Pathology. 2011 45()
Park JH et al., J Korean Neurosurg Soc 2012 (PMID:23115670)
Chien HC et al., PLoS One 2012 (PMID:23071813)
Immunofluorescent Localization of Tubulin
antibody to tubulin) FITC-labeled goat anti-rabbit (or secondary antibody to match the primary) Fluorescence Mounting Media Fluorescent Microscope equipped with 490 nm excitation filter and 515 nmbarrier filter Kodachrome film
remove the supernatants from the pellets, which are sometimes loose, and pool in a graduated cylinder. Note the volume (V2). 16. Add an equal volume of PM-8M, mix gently and store at -20°C. The tubulin at this stage
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
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