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- 技术资料
- 规格：
100 ug
predicted_size：~42 kDa
Uniprot ID：Q99788
description：CMKLR1 rabbit polyclonal antibody, Aff - Purified
clonality：Polyclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...)：Primary Antibodies
background：Chemokine receptor-like 1 (also designated G protein-coupled receptor DEZ or ChemR23) belongs to the G protein-coupled receptor 1 family. It is an integral membrane protein functioning as a receptor, possibly a chemotactic peptide receptor. It also acts as a co-receptor for various SIV strains and for a primary HIV-1 strain. ChemR23 is highly expressed in developing osseous and cartilaginous tissue, brain, kidney, gastrointestinal tissues and myeloid tissue, as well as in adult parathyroid glands.
immunogen：Synthetic peptide, corresponding to amino acids 216-265 of Human MChemR23.
recommended_dilution：Western blot: 1/500-1/1000. Immunofluorescence: 1/50-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/200.
predicted_size：~42 kDa
buffer：Phosphate buffered saline (PBS), pH 7.2.; State: Aff - Purified; State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE); Preservative: 0.05% Sodium Azide
host_species：Rabbit
applications：ELISA, IF, IHC, WB
reactivities：Human, Mouse, Rat
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文献和实验Use of EIA to Measure MMPs and TIMPs
the combination of a monoclonal antibody as the solid-phase with rabbit polyclonal antibody and horseradish peroxidase (HRP)-labeled donkey antirabbit gamma globulin as the second phase (2 ). EIAs for human MMP-9 were developed using MMP-9 specific polyclonal
Tips and hints for the storage of antibodies
! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses. Rabbit antisera Antisera are more robust than monoclonals. With anti-microbials added, they may be stored at ℃. Serum does not contain active proteases
Immunocytology using the HA epitope
. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.
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