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30 ul
predicted_size:null
Uniprot ID:P3481
description:CD68 mouse monoclonal antibody,clone UMAB150
clonality:Monoclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...):Primary Antibodies
background:This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]
immunogen:Human recombinant protein fragment corresponding to amino acids 22-319 of human CD68 (NP_001242)produced in SF9 cell.
recommended_dilution:WB 1:2000, IHC 1:100~200
predicted_size:null
buffer:PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
host_species:Mouse
applications:10k-ChIP, IHC, WB
reactivities:Human
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
(1)Cell Fusion and Selection Solutions: - IMDM - IMDM complete + 15% MCM. - PBS - PEG 50% ( w/v ): PEG 4000 ( 50% ) from Boehringer Manheim 1 243 268 - Aminopterin: Sigma, A 5159 (50X ) Prepare a T-150 flask with 170ml of IMDM
monoclonal antibody production fusion
Fusion is an important procedure to produce monoclonal antibodies (MoAb). PROTOCOL prepare the culture of myeloma cells and maintain the concentration of the cells at the level of less than 10
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