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- UM800144
- 2025年07月15日
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- 规格：
100 ul
predicted_size：33.2 kDa
Uniprot ID：O75144
description：ICOSLG (B7H2, CD275) mouse monoclonal antibody,clone UMAB254
clonality：Monoclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...)：Primary Antibodies
background：null
immunogen：Full length human recombinant protein of human ICOSLG (NP_056074) produced in HEK293T cell.
recommended_dilution：WB 1:1000, IHC 1:100~300
predicted_size：33.2 kDa
buffer：PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
host_species：Mouse
applications：10k-ChIP, IHC, WB
reactivities：Human
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
monoclonal antibody production fusion
Fusion is an important procedure to produce monoclonal antibodies (MoAb). PROTOCOL prepare the culture of myeloma cells and maintain the concentration of the cells at the level of less than 10
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