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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20度
- 克隆性:
多克隆
- 浓度:
2mg/1ml
- 抗体英文名:
Donkey Anti-rabbit IgG
- 规格:
1mg
| 英文名称 | Donkey Anti-rabbit IgG |
| 中文名称 | 驴抗兔IgG |
| 抗体来源 | Donkey |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rb |
| 产品应用 | WB=1:1000-5000 ELISA=1:2000-10000 IHC-P=1:200-1000 IHC-F=1:200-1000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 150kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 10mg/1ml |
| 免 疫 原 | Full length plasma protein:full length |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M PBS(pH7.4) |
| 保存条件 | Storage: Store at –20 oC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20oC. When reconstituted in sterile distilled water or diluent supplied, theantibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 兔IgG 定量 EIA 试剂盒使用说明 原理 本实验采用双抗体夹心 ELISA 法。用抗兔 IgG 单抗包被于酶标板上,标准品和样品中的 IgG 与单抗结合,加入酶标抗体,形成免疫复合物连接在板上,加入酶底物TMB ,出现蓝色,加终止液硫酸,颜色变黄,在 4 50 nm 处测 OD 值
抗TNF的抗血清(4)二抗:125I标记的驴抗兔IgG(5)未免疫兔血清(6)制备好的巨噬细胞悬液(细胞浓度为1×106ml)(7)96孔PVC板(聚本乙稀板)(8)γ-计数仪,温浴箱,刻度吸管,毛细吸管,加样器(头)三、操作步骤(1)将准备好的巨噬细胞悬液加入PVC板各孔中,0.1ml/孔,再用1%多聚甲醛固定于PVC板中,然后用PBS洗去多余的多聚甲醛;(2)设立不同倍比稀释度的TNF标准品;(3)加入3%BSA-PBS,每孔0.1ml, 37℃孵育1h,以封闭抗体非特异性结合位点;(3)加入3%BSA
R&D Systems 适用于神经细胞培养的无血清培养基添加剂
。 海马神经元表现出突触标记的可靠表达。e18大鼠海马神经元用添加了N21-MaX Media Supplement(目录号ar008)的培养基在体外培养21天。(a) 利用小鼠抗大鼠突触结合蛋白-1单克隆抗体(目录号MaB4364),以及Northernlights™ 557结合的驴抗小鼠igg二抗(目录号Nl007;黄色)对细胞染色,或(B) 利用小鼠抗人caM激酶iiα单克隆抗体(目录号MaB5584),接着用Northernlights™ 557结合的驴抗小鼠 igg二抗(目录号Nl
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