相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20度
- 克隆性:
多克隆
- 浓度:
2mg/1ml
- 抗体英文名:
Rabbit Anti-Biotin/Bio
- 规格:
100ul
| 英文名称 | Rabbit Anti-Biotin/Bio |
| 中文名称 | 生物素标记的兔抗生物素抗体IgG |
| 别 名 | Vitamin B4. |
| 产品类型 | 药物与化合物抗体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | biotin |
| 产品应用 | WB=1:1000-10000 ELISA=1:1000-10000 IHC-P=1:100-1000 IHC-F=1:100-1000 IF=1:100-1000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 2mg/1ml |
| 免 疫 原 | Full length plasma protein: |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile distilled water or diluent supplied, theantibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用。 8. 在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。
结合(详见参考文献1); 7. 交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用; 8. 在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。
或亲合素。所以卵白素―生物素免疫染色系统又称为抗生物素―生物素免疫染色法。 二、几中抗生物素―生物素染色法 1.抗生物素―生物素―过氧化酶复合物技术(Avidin Biotin �Peroxidase Complex technique, 简称ABC技术) (1)基本原理:ABC法是Hsu 等于1981年在HRAB法和LAB法的基础上改良的,其特点是利用抗生物素分别连接生物素标记的第二抗体和生物素标记的酶。与LAB法和BRAB法不同的是第一抗体不为标记物所标记,生物素标记
技术资料暂无技术资料 索取技术资料
生物素标记的兔抗生物素抗体IgG
¥428






