菌株简介: Donor strain (mobilizing strain) carrying the transfer genes of the broad host range IncP type plasmid RP4 integrated in its chromosome.Useful for mobilizing mobRP4 plasmids.The presence of λpir allows the replication of a defective R6K suicide plasmid and derivatives thereo.E. coli SM10 λpir strain contains the pir gene (lysogenized with λpir phage) and it is designed for cloning and propagation of plasmids with R6K origin of replication sequence (such as pUT mini-Tn5 vectors). The plasmids containing the R6K origin of replication require the R6K-spicific replication protein lpir gene for propagation. The genotype of the strain is KmR, thi-1, thr, leu, tonA, lacY, supE, recA::RP4-2-Tc::Mu, pir.
注意事项: 1、为了您的安全和健康,请穿实验服并戴一次性手套操作。本产品仅可用于实验室研究,不能用于动物,人体以及作为食品添加剂等用途。
2、使用甘油菌时可不完全融解,在甘油菌表面蘸取少量涂布固体琼脂平板即可,也可完全融解后使用,但随着冻融次数的增加,菌株的活力会逐渐下降。