YCPlac33酿酒酵母质粒

基本信息

原核抗性:	Amp
筛选标记:	URA3
克隆菌株:	DH5α
培养条件:	LB/37℃

质粒简介

          YCPlac33酿酒酵母质粒

质粒图谱

质粒序列

LOCUS       Exported                5601 bp ds-DNA     circular SYN 26-JUN-2018
DEFINITION  synthetic circular DNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    106..127
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        142..172
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..220
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             216..521
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /note="lacZ-alpha"
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIIAGPFS"
     misc_feature    233..289
                     /note="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(290..306)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1063..2225
                     /note="CEN/ARS"
                     /note="S. cerevisiae CEN4 centromere fused to the 
                     autonomously replicating sequence ARS1/ARS416"
     rep_origin      1388..2225
                     /note="ARS1"
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
     CDS             complement(2560..3363)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /note="URA3"
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(3364..3579)
                     /gene="S. cerevisiae URA3"
                     /note="URA3 promoter"
     promoter        3695..3799
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             3800..4660
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4831..5419
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
      241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg
      301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
      361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
      421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
      481 gcggtatttc acaccgcata atcgctgggc cattctcatg aagaatatct tgaatttatt
      541 gtcatattac tagttggtgt ggaagtccta atatcggtga tcaatatagt ggttgacatg
      601 ctggctagtc aacattgagc cttttgatca tgcaaatata ttacggtatt ttacaatcaa
      661 atatcaaact taactattga ctttataact tatttaggtg gtaacattct tataaaaaag
      721 aaaaaaatta ctgcaaaaca gtactagctt ttaacttgta tcctaggtta tctatgctgt
      781 ctcaccatag agaatattac ctatttcaga atgtatgtcc atgattcgcc gggtaaatac
      841 atataataca caaatctggc ttaataaagt ctataatata tctcataaag aagtgctaaa
      901 ttggctagtg ctatatattt ttaagaaaat ttcttttgac taagtccata tcgactttgt
      961 aaaagttcac attagcatac atatattaca cgagccagaa atagtaactt ttgcctaaat
     1021 cacaaattgc aaaatttaat tgcttgcaaa aggtcacatg cttataatca acttttttaa
     1081 aaatttaaaa tactttttta ttttttattt ttaaacataa atgaaataat ttatttattg
     1141 tttatgatta ccgaaacata aaacctgctc aagaaaaaga aactgttttg tccttggaaa
     1201 aaaagcacta cctaggagcg gccaaaatgc cgaggctttc atagcttaaa ctctttacag
     1261 aaaataggca ttatagatca gttcgagttt tcttattctt ccttccggtt ttatcgtcac
     1321 agttttacag taaataagta tcacctctta gagttcgatg ataagctgtc aaacatgaga
     1381 attaattcca catgttaaaa tagtgaagga gcatgttcgg cacacagtgg accgaacgtg
     1441 gggtaagtgc actagggtcc ggttaaacgg atctcgcatt gatgaggcaa cgctaattat
     1501 caacatatag attgttatct atctgcatga acacgaaatc tttacttgac gacttgaggc
     1561 tgatggtgtt tatgcaaaga aaccactgtg tttaatatgt gtcactgttt gatattactg
     1621 tcagcgtaga agataatagt aaaagcggtt aataagtgta tttgagataa gtgtgataaa
     1681 gtttttacag cgaaaagacg ataaatacaa gaaaatgatt acgaggatac ggagagaggt
     1741 atgtacatgt gtatttatat actaagctgc cggcggttgt ttgcaagacc gagaaaaggc
     1801 tagcaagaat cgggtcattg tagcgtatgc gcctgtgaac attctcttca acaagtttga
     1861 ttccattgcg gtgaaatggt aaaagtcaac cccctgcgat gtatattttc ctgtacaatc
     1921 aatcaaaaag ccaaatgatt tagcattatc tttacatctt gttattttac agattttatg
     1981 tttagatctt ttatgcttgc ttttcaaaag gcttgcaggc aagtgcacaa acaatactta
     2041 aataaatact actcagtaat aacctatttc ttagcatttt tgacgaaatt tgctattttg
     2101 ttagagtctt ttacaccatt tgtctccaca cctccgctta catcaacacc aataacgcca
     2161 tttaatctaa gcgcatcacc aacattttct ggcgtcagtc caccagctaa cataaaatgt
     2221 aagctctcgg ggctctcttg ccttccaacc cagtcagaaa tcgagttcca atccaaaagt
     2281 tcacctgtcc cacctgcttc tgaatcaaac aagggaataa acgaatgagg tttctgtgaa
     2341 gctgcactga gtagtatgtt gcagtctttt ggaaatacga gtcttttaat aactggcaaa
     2401 ccgaggaact cttggtattc ttgccacgac tcatctccat gcagttggac gatcgatgat
     2461 aagctgtcaa acatgagaat tgggtaataa ctgatataat taaattgaag ctctaatttg
     2521 tgagtttagt atacatgcat ttacttataa tacagttttt tagttttgct ggccgcatct
     2581 tctcaaatat gcttcccagc ctgcttttct gtaacgttca ccctctacct tagcatccct
     2641 tccctttgca aatagtcctc ttccaacaat aataatgtca gatcctgtag agaccacatc
     2701 atccacggtt ctatactgtt gacccaatgc gtctcccttg tcatctaaac ccacaccggg
     2761 tgtcataatc aaccaatcgt aaccttcatc tcttccaccc atgtctcttt gagcaataaa
     2821 gccgataaca aaatctttgt cgctcttcgc aatgtcaaca gtacccttag tatattctcc
     2881 agtagatagg gagcccttgc atgacaattc tgctaacatc aaaaggcctc taggttcctt
     2941 tgttacttct tctgccgcct gcttcaaacc gctaacaata cctgggccca ccacaccgtg
     3001 tgcattcgta atgtctgccc attctgctat tctgtataca cccgcagagt actgcaattt
     3061 gactgtatta ccaatgtcag caaattttct gtcttcgaag agtaaaaaat tgtacttggc
     3121 ggataatgcc tttagcggct taactgtgcc ctccatggaa aaatcagtca agatatccac
     3181 atgtgttttt agtaaacaaa ttttgggacc taatgcttca actaactcca gtaattcctt
     3241 ggtggtacga acatccaatg aagcacacaa gtttgtttgc ttttcgtgca tgatattaaa
     3301 tagcttggca gcaacaggac taggatgagt agcagcacgt tccttatatg tagctttcga
     3361 catgatttat cttcgtttcc tgcatgtttt tgttctgtgc agttgggtta agaatactgg
     3421 gcaatttcat gtttcttcaa cactacatat gcgtatatat accaatctaa gtctgtgctc
     3481 cttccttcgt tcttccttct gttcggagat taccgaatca aaaaaatttc aaagaaaccg
     3541 aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaat tgaaaagcta attcttgaag
     3601 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     3661 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     3721 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     3781 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     3841 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     3901 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     3961 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4021 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     4081 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4141 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     4201 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     4261 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     4321 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     4381 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     4441 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     4501 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     4561 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     4621 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     4681 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     4741 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     4801 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     4861 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     4921 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     4981 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5041 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5101 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5161 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5221 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     5281 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     5341 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     5401 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     5461 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     5521 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     5581 agtgagcgag gaagcggaag a