pCXSN-HA植物表达质粒

基本信息

启动子:	CaMV 35S promoter
终止子:	NOS terminator
质粒大小:	10832bp
质粒标签:	N-HA
原核抗性:	卡那霉素Kan
筛选标记:	潮霉素Hyg
克隆菌株:	大肠杆菌DB3.1
培养条件:	37℃，有氧，LB

质粒简介

         pCXSN-HA质粒是一个植物过表达质粒，35S启动子驱动HA和目的基因融合表达，在ccdb致死基因的作用下，克隆基因变得更加容易，阳性率高。

质粒图谱

质粒序列

LOCUS       Exported               10832 bp ds-DNA     circular SYN 30-OCT-2017
DEFINITION  synthetic circular DNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10832)
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10832
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      7..270
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             627..932
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(988..1014)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(1032..1922)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    2156..2180
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3479..4108
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4542..5609
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      5675..5869
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6213..6353
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6539..7127)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7214..8008)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8433..8457
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    8535..8709
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8749..9774)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(9841..10518)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 gaattccgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc
       61 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc
      121 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac
      181 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat
      241 tgccaaatgt ttgaacgatc ggggaaattc gctagtggat ccccaatact tgtatggggc
      301 ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata
      361 tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta
      421 cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc
      481 cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa
      541 agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt
      601 tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga
      661 gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga
      721 tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc
      781 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc
      841 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
      901 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt
      961 ctgcaggtcg acccatacaa gtattggagc gtaatctgga acatcgtatg ggtacatgga
     1021 tccccctcga gtatcgttcg taaatggtga aaattttcag aaaattgctt ttgctttaaa
     1081 agaaatgatt taaattgctg caatagaagt agaatgcttg attgcttgag attcgtttgt
     1141 tttgtatatg ttgtgttgag gtcgaggtcc tctccaaatg aaatgaactt ccttatatag
     1201 aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat
     1261 cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc
     1321 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct
     1381 ttcctttatc gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa
     1441 taaagtgaca gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt
     1501 gaaaagtctc aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga
     1561 caagtgtgtc gtgctccacc atgttatcac atcaatccac ttgctttgaa gacgtggttg
     1621 gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt
     1681 cggcagaggc atcttcaacg atggcctttc ctttatcgca atgatggcat ttgtaggagc
     1741 caccttcctt ttccactatc ttcacaataa agtgacagat agctgggcaa tggaatccga
     1801 ggaggtttcc ggatattacc ctttgttgaa aagtctcaat tgccctttgg tcttctgaga
     1861 ctgtatcttt gatatttttg gagtagacaa gtgtgtcgtg ctccaccatg ttgacctgca
     1921 ggcatgcaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     1981 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     2041 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgctagagca
     2101 gcttgagctt ggatcagatt gtcgtttccc gccttcagtt taaactatca gtgtttgaca
     2161 ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataac ggatatttaa
     2221 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc
     2281 ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag tcggcttctg
     2341 acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca
     2401 ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta
     2461 gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct
     2521 gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact
     2581 gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg
     2641 cccggagctg gccaggatgc ttgaccacct agccctggcg acgttgtgac agtgaccagg
     2701 ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag
     2761 gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc
     2821 cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac
     2881 cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct
     2941 accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc
     3001 gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag
     3061 cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca
     3121 ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga
     3181 aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga
     3241 tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg
     3301 aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg
     3361 aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc
     3421 atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat
     3481 gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca
     3541 tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca
     3601 gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat
     3661 cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat
     3721 cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt
     3781 gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct
     3841 ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg
     3901 ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct
     3961 gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg
     4021 cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc
     4081 cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc
     4141 acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc
     4201 agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac
     4261 accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa
     4321 tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc
     4381 ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc
     4441 catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgtct gccggccctg
     4501 caatggcact ggaaccccca agcccgagga atcggcgtga cggtcgcaaa ccatccggcc
     4561 cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gccgcgcagg
     4621 ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg
     4681 ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga
     4741 agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca
     4801 cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac
     4861 gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc
     4921 cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa
     4981 ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc
     5041 cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg
     5101 acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga
     5161 aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca
     5221 agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga
     5281 tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact
     5341 ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca
     5401 aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt
     5461 tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg
     5521 atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga
     5581 tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc
     5641 tagcagggga aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac attgggaacc
     5701 caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga
     5761 accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa
     5821 actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc
     5881 agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc
     5941 ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct ggcctacggc
     6001 caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac
     6061 atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     6121 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     6181 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat
     6241 agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     6301 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
     6361 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     6421 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     6481 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     6541 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     6601 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     6661 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     6721 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     6781 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     6841 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     6901 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     6961 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     7021 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     7081 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     7141 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     7201 tgcattctag gtactaaaac aattcatcca gtaaaatata atattttatt ttctcccaat
     7261 caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccgatatcct
     7321 ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg ccgcttctcc
     7381 caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg tctcccaggt
     7441 cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct
     7501 cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca tcggccagat
     7561 cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc gtatagggac
     7621 aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc tcgataatct
     7681 tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg gcctcactca
     7741 tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga acaggcagct
     7801 ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg gtccctttat
     7861 accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccagc ttatatacct
     7921 tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc agttttttca
     7981 attccggtga tattctcatt ttagccattt attatttcct tcctcttttc tacagtattt
     8041 aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt
     8101 ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa
     8161 catagtatcg acggagccga ttttgaaacc gcggtgatca caggcagcaa cgctctgtca
     8221 tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc cggcagctta
     8281 gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct tacaacggct
     8341 ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg
     8401 agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa
     8461 ttgacgctta gacaacttaa taacacattg cggacgtttt taatgtactg aattaacgcc
     8521 gaattaattc gggggatctg gattttagta ctggattttg gttttaggaa ttagaaattt
     8581 tattgataga agtattttac aaatacaaat acatactaag ggtttcttat atgctcaaca
     8641 catgagcgaa accctatagg aaccctaatt cccttatctg ggaactactc acacattatt
     8701 atggagaaac tcgagcttgt cgatcgacag atccggtcgg catctactct atttctttgc
     8761 cctcggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta cacagccatc
     8821 ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga
     8881 tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac
     8941 caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga gtcgtggcga
     9001 tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa
     9061 ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga acatcgcctc
     9121 gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt tggagccgaa
     9181 atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca tcagctcatc
     9241 gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc agtgatacac
     9301 atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac cgattccttg
     9361 cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga tcgcatccat
     9421 agcctccgcg accggttgta gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt
     9481 gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctaaact ccccaatgtc
     9541 aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat aacgatcttt
     9601 gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc ctacatcgaa
     9661 gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaga cgctgtcgaa
     9721 cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt tcatatctca
     9781 ttgccccccg gatctgcgaa agctcgagag agatagattt gtagagagag actggtgatt
     9841 tcagcgtgtc ctctccaaat gaaatgaact tccttatata gaggaaggtc ttgcgaagga
     9901 tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc cacttgcttt
     9961 gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct
    10021 ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc gcaatgatgg
    10081 catttgtagg tgccaccttc cttttctact gtccttttga tgaagtgaca gatagctggg
    10141 caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc aatagccctt
    10201 tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc gtgctccacc
    10261 atgttatcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg
    10321 atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaacg
    10381 atagcctttc ctttatcgca atgatggcat ttgtaggtgc caccttcctt ttctactgtc
    10441 cttttgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc
    10501 ctttgttgaa aagtctcaat agccctttgg tcttctgaga ctgtatcttt gatattcttg
    10561 gagtagacga gagtgtcgtg ctccaccatg ttggcaagct gctctagcca atacgcaaac
    10621 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
    10681 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
    10741 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
    10801 ttcacacagg aaacagctat gaccatgatt ac