pCXSN-FLAG植物表达质粒

基本信息

启动子:	CaMV 35S promoter
终止子:	NOS terminator
质粒大小:	10829bp
质粒标签:	N-FLAG
原核抗性:	卡那霉素Kan
筛选标记:	潮霉素Hyg
克隆菌株:	大肠杆菌DB3.1
培养条件:	37℃，有氧，LB

质粒简介

            pCXSN-FLAG质粒是一个植物过表达质粒，35S启动子驱动FLAG标签和目的基因融合表达，在ccdb致死基因的作用下，克隆基因变得更加容易，阳性率高。

质粒图谱

质粒序列

LOCUS       Exported               10829 bp ds-DNA     circular SYN 30-OCT-2017
DEFINITION  synthetic circular DNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10829)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10829
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      7..270
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             627..932
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(988..1011)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(1029..1919)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    2153..2177
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3476..4105
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4539..5606
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      5672..5866
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6210..6350
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6536..7124)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7211..8005)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8430..8454
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    8532..8706
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8746..9771)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(9838..10515)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 gaattccgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc
       61 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc
      121 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac
      181 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat
      241 tgccaaatgt ttgaacgatc ggggaaattc gctagtggat ccccaatact tgtatggggc
      301 ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata
      361 tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta
      421 cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc
      481 cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa
      541 agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt
      601 tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga
      661 gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga
      721 tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc
      781 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc
      841 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
      901 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt
      961 ctgcaggtcg acccatacaa gtattggctt atcatcatca tccttgtaat ccatggatcc
     1021 ccctcgagta tcgttcgtaa atggtgaaaa ttttcagaaa attgcttttg ctttaaaaga
     1081 aatgatttaa attgctgcaa tagaagtaga atgcttgatt gcttgagatt cgtttgtttt
     1141 gtatatgttg tgttgaggtc gaggtcctct ccaaatgaaa tgaacttcct tatatagagg
     1201 aagggtcttg cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac
     1261 atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg
     1321 tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttcaacg atggcctttc
     1381 ctttatcgca atgatggcat ttgtaggagc caccttcctt ttccactatc ttcacaataa
     1441 agtgacagat agctgggcaa tggaatccga ggaggtttcc ggatattacc ctttgttgaa
     1501 aagtctcaat tgccctttgg tcttctgaga ctgtatcttt gatatttttg gagtagacaa
     1561 gtgtgtcgtg ctccaccatg ttatcacatc aatccacttg ctttgaagac gtggttggaa
     1621 cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg
     1681 cagaggcatc ttcaacgatg gcctttcctt tatcgcaatg atggcatttg taggagccac
     1741 cttccttttc cactatcttc acaataaagt gacagatagc tgggcaatgg aatccgagga
     1801 ggtttccgga tattaccctt tgttgaaaag tctcaattgc cctttggtct tctgagactg
     1861 tatctttgat atttttggag tagacaagtg tgtcgtgctc caccatgttg acctgcaggc
     1921 atgcaagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     1981 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     2041 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgc tagagcagct
     2101 tgagcttgga tcagattgtc gtttcccgcc ttcagtttaa actatcagtg tttgacagga
     2161 tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataacgga tatttaaaag
     2221 ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc
     2281 tcgggatcaa agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg
     2341 ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc
     2401 tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa
     2461 tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct
     2521 gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca
     2581 cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc
     2641 ggagctggcc aggatgcttg accacctagc cctggcgacg ttgtgacagt gaccaggcta
     2701 gaccgcctgg cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc
     2761 ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc
     2821 atggtgttga ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc
     2881 acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc ccgccctacc
     2941 ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg
     3001 aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc
     3061 agcgaggaag tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg
     3121 accgaggccg acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac
     3181 cgcaccagga cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga
     3241 tcgcggccgg gtacgtgttc gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa
     3301 tcctggccgg tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag
     3361 aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg
     3421 cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa
     3481 ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct
     3541 agcccgcgcc ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg
     3601 cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga
     3661 ccgcccgacg attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga
     3721 cggagcgccc caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct
     3781 gattccggtg cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt
     3841 taagcagcgc attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc
     3901 gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc
     3961 cattcttgag tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac
     4021 aaccgttctt gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc
     4081 tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca
     4141 aacacgctaa gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc
     4201 ctggcagaca cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc
     4261 aagctgaaga tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac
     4321 atcgcgcagc taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc
     4381 taaaggaggc ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat
     4441 gtgtggagga acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa
     4501 tggcactgga acccccaagc ccgaggaatc ggcgtgacgg tcgcaaacca tccggcccgg
     4561 tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg
     4621 cccagcggca acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg
     4681 atcgaatccg caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc
     4741 cgcccaaggg cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc
     4801 gcgatagtcg cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag
     4861 ctggcgaggt gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg
     4921 ccggcatggc cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg
     4981 aatccatgaa ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac
     5041 acgttgcgga cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc
     5101 tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg
     5161 ccaagaacgg ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga
     5221 tcgtaaagag cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt
     5281 accgcgagat cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt
     5341 tgatcgatcc cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg
     5401 cagaagccag atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca
     5461 agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt
     5521 tgaaggagga ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg
     5581 agggcgaagc atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag
     5641 caggggaaaa aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa
     5701 agccgtacat tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc
     5761 ggtcacacat gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact
     5821 ctttaaaact tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc
     5881 gcacagccga agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg
     5941 ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag
     6001 gcaatctacc agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc
     6061 aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     6121 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     6181 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc
     6241 ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     6301 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg
     6361 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     6421 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     6481 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6541 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6601 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6661 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6721 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6781 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6841 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6901 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6961 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     7021 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     7081 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     7141 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc
     7201 attctaggta ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag
     7261 gcttgatccc cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc
     7321 tgatcgaccg gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa
     7381 gatcaataaa gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc
     7441 cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc
     7501 gcggatcttt aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt
     7561 tattcagtaa gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat
     7621 ccgatatgtc gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt
     7681 cagggctttg ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga
     7741 gcagattgct ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc
     7801 cttccagcca tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc
     7861 ggctgtccgt catttttaaa tataggtttt cattttctcc caccagctta tataccttag
     7921 caggagacat tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt
     7981 ccggtgatat tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa
     8041 gataccccaa gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta
     8101 aaaccttaaa taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat
     8161 agtatcgacg gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg
     8221 ttacaatcaa catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt
     8281 gccgttcttc cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc
     8341 ccgctgacgc cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc
     8401 tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg
     8461 acgcttagac aacttaataa cacattgcgg acgtttttaa tgtactgaat taacgccgaa
     8521 ttaattcggg ggatctggat tttagtactg gattttggtt ttaggaatta gaaattttat
     8581 tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat
     8641 gagcgaaacc ctataggaac cctaattccc ttatctggga actactcaca cattattatg
     8701 gagaaactcg agcttgtcga tcgacagatc cggtcggcat ctactctatt tctttgccct
     8761 cggacgagtg ctggggcgtc ggtttccact atcggcgagt acttctacac agccatcggt
     8821 ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg ctccggatcg
     8881 gacgattgcg tcgcatcgac cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa
     8941 gctctgatag agttggtcaa gaccaatgcg gagcatatac gcccggagtc gtggcgatcc
     9001 tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac aagccaacca
     9061 cggcctccag aagaagatgt tggcgacctc gtattgggaa tccccgaaca tcgcctcgct
     9121 ccagtcaatg accgctgtta tgcggccatt gtccgtcagg acattgttgg agccgaaatc
     9181 cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc caaagcatca gctcatcgag
     9241 agcctgcgcg acggacgcac tgacggtgtc gtccatcaca gtttgccagt gatacacatg
     9301 gggatcagca atcgcgcata tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg
     9361 tccgaatggg ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg catccatagc
     9421 ctccgcgacc ggttgtagaa cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac
     9481 accctgtgca cggcgggaga tgcaataggt caggctctcg ctaaactccc caatgtcaag
     9541 cacttccgga atcgggagcg cggccgatgc aaagtgccga taaacataac gatctttgta
     9601 gaaaccatcg gcgcagctat ttacccgcag gacatatcca cgccctccta catcgaagct
     9661 gaaagcacga gattcttcgc cctccgagag ctgcatcagg tcggagacgc tgtcgaactt
     9721 ttcgatcaga aacttctcga cagacgtcgc ggtgagttca ggctttttca tatctcattg
     9781 ccccccggat ctgcgaaagc tcgagagaga tagatttgta gagagagact ggtgatttca
     9841 gcgtgtcctc tccaaatgaa atgaacttcc ttatatagag gaaggtcttg cgaaggatag
     9901 tgggattgtg cgtcatccct tacgtcagtg gagatatcac atcaatccac ttgctttgaa
     9961 gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg
    10021 ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat
    10081 ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa
    10141 tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg
    10201 tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg
    10261 ttatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg
    10321 ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata
    10381 gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt
    10441 ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt
    10501 tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag
    10561 tagacgagag tgtcgtgctc caccatgttg gcaagctgct ctagccaata cgcaaaccgc
    10621 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
    10681 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
    10741 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
    10801 acacaggaaa cagctatgac catgattac