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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20度
- 保质期：
2年
- 供应商：
钦诚生物
- 规格：
5ug
基本信息
启动子: CMV promoter
复制子: pUC ori,f1 ori,SV40 ori
终止子: SV40 poly(A) signal
质粒大小: 5113bp
质粒标签: N-EGFP
原核抗性: 卡那霉素Kan
筛选标记: 新霉素Neo/G418/EGFP
克隆菌株: 大肠杆菌DH5α
培养条件: 37℃，有氧，LB
表达宿主: 哺乳细胞293T
诱导方式: 无须诱导，瞬时表达
5'测序引物: CMV-F：CGCAAATGGGCGGTAGGCGTG
3'测序引物: Sv40-polyA-R：GAAATTTGTGATGCTATTGC
质粒简介
pEGFP-LC3b质粒是一个哺乳细胞过表达质粒，CMV启动子驱动EGFP绿色荧光蛋白基因和LC3b人源基因融合表达，可用于细胞自噬研究。
质粒图谱
质粒序列
LOCUS Exported 5113 bp ds-DNA circular SYN 29-DEC-2015
DEFINITION synthetic circular DNA
KEYWORDS pEGFP-LC3b-h
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5113)
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..5113
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 61..364
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 613..1329
/codon_start=1
/product="enhanced GFP"
/note="EGFP"
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
misc_feature 1363..1740
/note="LC3B-h"
polyA_signal 1901..2022
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(2029..2484)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2511..2615
/gene="bla"
/note="AmpR promoter"
promoter 2617..2974
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2825..2960
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 3009..3803
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 4035..4082
/note="HSV TK poly(A) signal"
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4411..4999
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
1321 ctgtacaagt actcagatct cgagctcaag cttcgaattc ccatgccgtc ggagaagacc
1381 ttcaagcagc gccgcacctt cgaacaaaga gtagaagatg tccgacttat tcgagagcag
1441 catccaacca aaatcccggt gataatagaa cgatacaagg gtgagaagca gcttcctgtt
1501 ctggataaaa caaagttcct tgtacctgac catgtcaaca tgagtgagct catcaagata
1561 attagaaggc gcttacagct caatgctaat caggccttct tcctgttggt gaacggacac
1621 agcatggtca gcgtctccac accaatctca gaggtgtatg agagtgagaa agatgaagat
1681 ggattcctgt acatggtcta tgcctcccag gagacgttcg ggatgaaatt gtcagtgtaa
1741 gaattctgca gtcgacggta ccgcgggccc gggatccacc ggatctagat aactgatcat
1801 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
1861 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
1921 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
1981 gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg
2041 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
2101 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
2161 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
2221 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
2281 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
2341 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
2401 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
2461 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
2521 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
2581 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg
2641 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
2701 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
2761 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
2821 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
2881 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
2941 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat
3001 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
3061 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
3121 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
3181 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
3241 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
3301 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
3361 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
3421 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
3481 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
3541 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
3601 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
3661 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
3721 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
3781 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
3841 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
3901 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
3961 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
4021 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
4081 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
4141 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
4201 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc
4261 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
4321 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
4381 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
4441 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
4501 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
4561 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
4621 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
4681 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
4741 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
4801 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
4861 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
4921 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
4981 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
5041 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
5101 taccgccatg cat
//
启动子: CMV promoter
复制子: pUC ori,f1 ori,SV40 ori
终止子: SV40 poly(A) signal
质粒大小: 5113bp
质粒标签: N-EGFP
原核抗性: 卡那霉素Kan
筛选标记: 新霉素Neo/G418/EGFP
克隆菌株: 大肠杆菌DH5α
培养条件: 37℃，有氧，LB
表达宿主: 哺乳细胞293T
诱导方式: 无须诱导，瞬时表达
5'测序引物: CMV-F：CGCAAATGGGCGGTAGGCGTG
3'测序引物: Sv40-polyA-R：GAAATTTGTGATGCTATTGC
质粒简介
pEGFP-LC3b质粒是一个哺乳细胞过表达质粒，CMV启动子驱动EGFP绿色荧光蛋白基因和LC3b人源基因融合表达，可用于细胞自噬研究。
质粒图谱
质粒序列
LOCUS Exported 5113 bp ds-DNA circular SYN 29-DEC-2015
DEFINITION synthetic circular DNA
KEYWORDS pEGFP-LC3b-h
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5113)
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..5113
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 61..364
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 613..1329
/codon_start=1
/product="enhanced GFP"
/note="EGFP"
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
misc_feature 1363..1740
/note="LC3B-h"
polyA_signal 1901..2022
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(2029..2484)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2511..2615
/gene="bla"
/note="AmpR promoter"
promoter 2617..2974
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2825..2960
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 3009..3803
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 4035..4082
/note="HSV TK poly(A) signal"
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4411..4999
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
1321 ctgtacaagt actcagatct cgagctcaag cttcgaattc ccatgccgtc ggagaagacc
1381 ttcaagcagc gccgcacctt cgaacaaaga gtagaagatg tccgacttat tcgagagcag
1441 catccaacca aaatcccggt gataatagaa cgatacaagg gtgagaagca gcttcctgtt
1501 ctggataaaa caaagttcct tgtacctgac catgtcaaca tgagtgagct catcaagata
1561 attagaaggc gcttacagct caatgctaat caggccttct tcctgttggt gaacggacac
1621 agcatggtca gcgtctccac accaatctca gaggtgtatg agagtgagaa agatgaagat
1681 ggattcctgt acatggtcta tgcctcccag gagacgttcg ggatgaaatt gtcagtgtaa
1741 gaattctgca gtcgacggta ccgcgggccc gggatccacc ggatctagat aactgatcat
1801 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
1861 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
1921 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
1981 gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg
2041 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
2101 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
2161 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
2221 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
2281 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
2341 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
2401 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
2461 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
2521 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
2581 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg
2641 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
2701 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
2761 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
2821 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
2881 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
2941 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat
3001 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
3061 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
3121 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
3181 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
3241 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
3301 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
3361 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
3421 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
3481 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
3541 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
3601 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
3661 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
3721 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
3781 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
3841 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
3901 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
3961 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
4021 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
4081 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
4141 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
4201 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc
4261 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
4321 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
4381 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
4441 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
4501 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
4561 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
4621 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
4681 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
4741 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
4801 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
4861 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
4921 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
4981 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
5041 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
5101 taccgccatg cat
//
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文献和实验相关实验
的。ATG5 在死亡肿瘤细胞中高表达，因此成为抗肿瘤治疗成功与否的重要指标，而 ATG12-ATG5-ATG16L 复合物的形成对自噬隔离膜的延伸是至关重要的。LC3 是 Atg8 在哺乳动物细胞中的同源物，LC3B-I(定位于胞浆) 与磷脂酰乙醇胺（PE）共价连接并插入到自噬体的双层膜中形成 LC3B II。LC3 偶联到自噬体上以及 LC3-I 转换成 LC3-II，已被用来作为自噬评价和测定的公认指标。自噬溶酶体形成自噬体与溶酶体的直接融合形成自噬溶酶体，形成过程中主要是 LAMP1、Rab
水平的高低。LC3 这一转变过程可被 Western Blot 和荧光显微镜检测到，现已成为监测自噬体形成的推荐方法。在哺乳动物中，LC3 包含 3 种亚型：LC3A、LC3B、LC3C，分布在不同的组织中，因此需要用不同的抗体去鉴定这3种亚型。 和元生物能够提供成熟的细胞自噬相关的技术服务 GFP-LC3 单荧光自噬指示体系： 利用 LC3 在自噬形成过程中发生聚集的现象构建了 GFP-LC3 指示体系，无自噬时，GFP-LC3 融合蛋白弥散在胞浆中；自噬形成时，GFP-LC3 融合
tianlang126 wrote: :(:(新手最近想做细胞自噬，想知道beclin1在自噬中起个什么样的作用？是不是表达beclin1就可以认为细胞发生了自噬？它和凋亡、坏死、炎症等有什么关系？它是通过什么信号通路起作用？ 希望各位大虾解解惑:8):8) 他是个明星分子 但是不是自噬的标志性分子 自噬的标志性分子是LC3 准确说是MAP1LC3B baker099 Beclin1在自噬的早期组装
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pEGFP-LC3b哺乳自噬质粒
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