CD3E (Cytopl. Dom.) rat monocl

gene_symbol：CD3E
predicted_size：null
Uniprot ID：P7766
description：CD3E (Cytopl. Dom.) rat monoclonal antibody, clone CD3-12, Purified
clonality：Monoclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...)：Primary Antibodies
background：T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
immunogen：Synthetic peptide sequence derived from Cytoplasmic epitope of CD3
recommended_dilution：Western Blot.  Flow Cytometry: 1/50-1/100 (Membrane permeabilisation is required). Immunohistochemistry on Frozen Sections: 1/1000.  Immunohistochemistry on Paraffin Embedded Tissues: 1/1000. Heat antigen retrieval treatment for 20 minutes prior to staining of paraffin sections is required (Tris/EDTA buffer pH 9.0 is recommended for this purpose). Recommended Positive Control Tissue: Tonsil.
predicted_size：null
buffer：PBS; State: Purified; State: Liquid purified IgG fraction from Tissue Culture Supernatant; Preservative: 0.09% Sodium Azide
host_species：Rat
applications：FC, IF, IHC, WB
reactivities：Canine, Chicken, Equine, Human, Mouse, Porcine