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- 2025年07月11日
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![AF10 (MLLT10) Rabbit Monoclonal Antibody [Clone ID: R07-2G2]](https://img1.dxycdn.com/p/s14/2025/0217/601/8427578297774731981.png!wh200)
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- 详细信息
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- 技术资料
- 规格：
100 ul
predicted_size：null
Uniprot ID
description：Clone OTI7E11, Anti-V5 tag monoclonal antibody
clonality：Monoclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...)：Tag Antibodies
background：null
immunogen：Anti-V5 monoclonal antibody is produced by immunizing mice with a synthetic peptide coupled to KLH
recommended_dilution：WB: 1:2000
predicted_size：null
buffer：null
host_species：Mouse
applications：WB
reactivities：V5 tag
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文献和实验Monoclonal Antibody Production
(note the removal of the HT)in a T25 flask (one for each positive clone)for later use (7) When the cells are ready for expansion out of the 24-well plate,resuspend them and pipet to the T25 flasks with feeder cells.Again leave a little bit in the 24
monoclonal antibody production fusion
Fusion is an important procedure to produce monoclonal antibodies (MoAb). PROTOCOL prepare the culture of myeloma cells and maintain the concentration of the cells at the level of less than 10
Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
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