Matrices listed below were spiked with certain level of FBLN1 and the recovery rates were calculated by comparing the measured value to the expected amount of FBLN1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-103
94
EDTA plasma(n=5)
87-102
97
heparin plasma(n=5)
89-98
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FBLN1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.