Matrices listed below were spiked with certain level of VKORC1 and the recovery rates were calculated by comparing the measured value to the expected amount of VKORC1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-102
95
EDTA plasma(n=5)
86-103
95
heparin plasma(n=5)
87-97
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VKORC1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.