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文献和实验Mouse B cell isolation with magnetic beads
, grind the tissue until no big tissue chunks are left and most cells have gone into suspension; 4.Rinse the strainer with MACS buffer, and pipette up and down the cell suspension to break cell aggregates; 5.Transfer cell suspension to 15ml
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
, horizontal Gel imaging system for quantifying PCR products Heat block preset to 65°C Hemocytometer Ice Incubator, humidified, equilibrated with 5% CO2 , preset to 37°C Liquid nitrogen (optional; see Step 11) Magnetic stand
Mag-Bind RX Plasmid Isolation Protocol
plate. 9. Add 10 ul Mag-Bind Particles SX followed by 70 ul PFC Buffer. Mix the sample throughly by pipetting up and down for 10 times. 10. Place the plate onto the magnetic separation stand to magnetite the magnetic particles. Remove
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