- 询价
- merck millipore
- JMCDF1L101
- 2025年07月16日
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文献和实验Microinjection DNA Purification
from the cartridge: Replace tube with a fresh microtube. Add 50 microliters of preheated (60 degrees Centigrade) elution buffer to cartridge. Spin at maximum speed for 60 seconds in a microcentrifuge. Elution buffer: 10 mM Tris-HCl, pH 8.5, 0.02 micron filtered
Microinjection DNA Purification
onto the cartridge. Spin at maximum speed for 10 seconds in a microcentrifuge. Reload the flowthrough and spin again. Discard the flowthrough after the second spin. The spin filter has a capacity of 20 micrograms DNA, so you can run several 700 microliters loads
Purification of Endotoxin Free mAbs
in endotoxin free water containing 10mM EDTA and subsequently stored sterile in endotoxin free water containing 0.1% sodium azide). Finally, centrifuge (at least 3500rpm for 30 min in bench centrifuge) and 0.2 micron sterile filter to remove any grossly
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