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- 2025年07月15日
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文献和实验Selection of Transfected Cells: Magnetic Affinity Cell Sorting
of stably transfected cells with a selectable marker gives rise to a cell population expressing the gene of interest free of untransfected cells under conditions of drug selection. However, this method of selection is a long-term process and may have adverse
Isolation of cells expressing transfected cDNAcan be extremely difficult and tedious if the efficiency of expression is low (less than 1–5%). If the receptor being selected for is a cell surface or integral membrane protein
Inducible Expression of G Protein-Coupled Receptors in Transfected Cells
Biochemical or pharmacological studies of G protein-coupled receptors (GPCRs) are widely conducted in transfected mammalian cells. A variety of commercially available systems allow the generation of stable cell-lines in which expression
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