Nitrotyrosine-modified BSA (po

Poly(ADP-ribose) monoclonal antibody (10H)产品货号ALX-804-220，由ENZO公司研发生产，欣博盛生物是ENZO公司授权一级代理商，如需购买ENZO公司产品，技术问题，请联系欣博盛生物。热销品常备现货~

The monoclonal antibody 10H is directed against poly(ADP-ribose) (PAR). PAR is synthesized after activation of the nuclear DNA repair enzyme poly(ADP-ribose)polymerase (PARP). PARP is selectively activated by DNA strand breaks to catalyze the addition of long branched chains of PAR to a variety of nuclear proteins, most notably PARP itself.The amount of PAR formed in living cells with DNA damage is commensurate with the extent of the damage. Under DNA damage conditions, PAR undergoes a rapid turnover, with a half-life in the range of minutes, as PAR is rapidly hydrolyzed and converted to free ADP-ribose by the enzyme poly(ADP-ribose)glycohydrolase (PARG). After massive DNA damage (e.g. γ-irradiation or oxidative stress) PAR is detectable in the first 10 minutes and disappears later on. In keratinocytes MAb 10H has been shown to detect UVB-induced apoptosis as early as 4 hour after irradiation, thus being superior to DNA laddering and the TUNEL assay.Due to the very large number of endonuclease-mediated DNA breaks in apoptosis, PARP becomes strongly activated during the so-called execution phase. In the case of DNA damage-induced apoptosis, this represents a “second round” of PAR synthesis. PAR synthesized during apoptosis appears to be remarkably stable. PAR immunofluorescence appears at least as early during apoptosis as does the specific cleavage of PARP by caspase-3. As shown by several groups, this PAR immunofluorescence correlates well with other markers of apoptosis. MAb to Poly(ADP-ribose) (10H) can be used in flow cytometry.A quantitative non-isotopic immuno-dot-blot method for the assessment of cellular poly(ADP-ribosyl)ation capacity using MAb to Poly(ADP-ribose) (10H) has been described.

Ecommerce is not available in your country. Please get in touch with your local distributor.

This antibody is covered by our Worry-Free Guarantee.

Product Details
Alternative Name    
PAR

Application    
Flow Cytometry, ICC, IHC (PS), WB

Clone    
10H

Formulation    
Liquid. In 50mM HEPES, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide.

Host    
Mouse

Immunogen    
Purified poly(ADP-ribose).

Isotype    
IgG3

Purity Detail    
Protein A-affinity purified from supernatant.

Recommendation Dilutions/Conditions    
Immunocytochemistry (5-20µg/ml)Immunohistochemistry (paraffin sections; dilution buffer: 5% milk (non fat dried milk) in PBS to a final concentration of 5-20µg/ml)Western Blot (incubate 2.5µg/ml in PBS, 0.05% Tween20, 5% milk (non fat dried milk))Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

Species Reactivity    
Drosophila, Human, Mouse, Rat

Specificity    
Recognizes poly(ADP-ribose) synthesized by a broad range of PARPs (poly(ADP-ribose) polymerases) like human, mouse, rat or Drosophila PARP enzyme.

Technical Info / Product Notes    
Cited samples:
For an overview on cited samples please click here.

Worry-free Guarantee    
This antibody is covered by our Worry-Free Guarantee.

Handling & Storage
Handling    
Avoid freeze/thaw cycles.

Long Term Storage    
-20°C

Shipping    
Blue Ice

Enzo Life Sciences, Inc.隶属于全球上市公司Enzo Biochem，Inc.，总部设于美国纽约的法明代尔。Enzo在研发、生产、销售生物研究试剂方面拥有30多年的丰富成功经验，可提供多种细胞活性荧光染料、蛋白、抗体、多肽、小分子、标签探针染料及试剂盒等，用于研究目标的鉴定、高通量分析、基因表达分析、核酸检测、蛋白组学研究及细胞检测等。