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- 详细信息
- 文献和实验
- 技术资料
- 样本:
血清/血浆/组织匀浆液
- 库存:
1000
- 适应物种:
Mouse
- 应用:
科研使用
- 检测方法:
ELISA
- 检测范围:
0.312 - 20 U/ml
- 供应商:
康朗生物
- 规格:
96T
Mouse IgG ELISA Kit (Sandwich ELISA)
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Product Description
LS-F54798 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse IgG in samples of Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of IgG as low as 0.078 units per milliliter.Specifications
TypeSandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
IgG
Reactivity
Mouse
Manual
Intended Sample Types
Plasma, Serum
Sample Size
50 - 100 µl
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
0.312 - 20 U/ml
Sensitivity
0.078 U/ml
Assay Time
4.5 h
Precision
Intra-Assay: CV<8% / Inter-Assay: CV<10%
Storage
Store at 2°C to 8°C. Do not use after expiration date. Maybe be stored for up to 1 month after opeing.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- User Manual
- 96-well Microplate
- Detection Reagents
- Wash Buffer
- Substrate
- Stop Solution
- Adhesive Plate Sealers
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
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人抗磷脂抗体IgG(APL-IgG)酶联免疫分析(ELISA)
人抗磷脂抗体IgG(APL-IgG) 酶联免疫分析(ELISA) 试剂盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆及相关液体样本中抗磷脂抗体IgG(APL-IgG) 的含量。 实验原理: 本试剂盒应用双抗原夹心法测定标本中人抗磷脂抗体 IgG(APL-IgG) 水平。用纯化的抗磷脂抗体 IgG(APL-IgG) 抗原包被微孔板,制成固相抗原,往包被抗原的微孔中依次加入抗磷脂抗体
可作夹心法的两个以上的位点,因此不能用双抗体夹心法进行测定,可以采用竞争法模式。其原理是标本中的抗原和一定量的酶标抗原竞争与固相抗体结合。标本中抗原量含量愈多,结合在固相上的酶标抗原愈少,最后的显色也愈浅。小分子激素、药物等ELISA测定多用此法。 6.捕获包被法测抗体 IgM抗体的检测用于传染病的早期诊断中。间接法ELISA一般仅适用于检测总抗体或IgG抗体。如用抗原包被的间接法直接测定IgM抗体,因标本中一般同时存在较高浓度的IgG抗体,后者将竞争结合固相抗原而使一部份IgM抗体不能结合
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