Matrices listed below were spiked with certain level of ITGAM and the recovery rates were calculated by comparing the measured value to the expected amount of ITGAM in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-105
93
EDTA plasma(n=5)
90-101
96
heparin plasma(n=5)
91-105
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ITGAM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.