- $590
- LSBio
- 1.5 - 400 ng/ml
- LS-F49875
- 美国
- 2025年07月12日
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- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Human
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
1.5 - 400 ng/ml
- 供应商：
康朗生物
- 规格：
96T
Human ITGAV/Integrin Alpha V/CD51 ELISA Kit (Sandwich ELISA)
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Product Description
LS-F49875 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human ITGAV/Integrin Alpha V/CD51 in samples of Cell Culture Supernatants, Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of ITGAV/Integrin Alpha V/CD51 as low as 1.5 nanograms per millilter.Specifications
TypeSandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
ITGAV/Integrin Alpha V/CD51
Reactivity
Human
Specificity
Human CD51
Manual
Intended Sample Types
Cell Culture Supernatants, Plasma, Serum
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
1.5 - 400 ng/ml
Sensitivity
1.5 ng/ml
Assay Time
4.5 h
Precision
Intra-Assay: CV<10% / Inter-Assay: CV<12%
Storage
Store at 4°C for 6 months or at -20°C for 1 year. Avoid freeze/thaw cycles.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- User Manual
- 96-well Microplate
- Detection Reagents
- Wash Buffer
- Substrate
- Stop Solution
- Adhesive Plate Sealers
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
About ITGAV/Integrin Alpha V/CD51
P06756 NM_002210 NP_002201.1
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Sandwich ELISA Platform Overview
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文献和实验调至 1μg/μl。 4. ELISA 检测：按试剂盒说明书完成板平衡、加样孵育、洗板、酶标抗体孵育、显色终止与读数，结合标准曲线得出 IFN-γ 浓度。 四、操作要点速记 1.组织样本新鲜处理，全程低温操作，裂解液现配现用； 2. 蛋白定量时标准品与样本同步孵育，稀释采用倍比稀释减少误差； 3. ELISA 实验需每次重绘标准曲线，洗板充分、严格把控显色与终止时间。 免疫检测试剂盒： 货号-规格 名称 abs510001-96T Human IL-2 ELISA Kit (Faster
一篇文献中的ELISA方法如下：Control peptides (14–17 amino acid sequence near the N-terminal or C-terminal of the studied protein) and the reactive rabbit anti-defensin antibodies (affinity pure) were obtained from Alpha Diagnostics .CoStar flat-bottom high
操作条件、人员一致 边缘效应 蒸发/酶标板叠放 孵育加封板膜或盖板；避免叠放 孵育温度不均 校准温育箱；避开通风口、窗边等环境波动区 洗板不彻底 确保洗板仪正常；不减少洗液量与次数 注意！！！ ELISA试剂盒11月活动即将来袭~敬请期待~ 部分热卖产品推荐 产品货号 产品名称 检测范围 灵敏度 97109ES Human IL18 ELISA Kit 125
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