Matrices listed below were spiked with certain level of GDF2 and the recovery rates were calculated by comparing the measured value to the expected amount of GDF2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
92-102
98
EDTA plasma(n=5)
85-102
93
heparin plasma(n=5)
87-103
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GDF2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.