Mouse/Human/Rat Phospho-IRS1 (

Mouse/Human/Rat Phospho-IRS1 (Ser307) ELISA Kit (Cell-Based Phosphorylation ELISA)

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LS-F1110-1
1 plate
$740 USD
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Product Description

LS-F1110 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Qualitative detection of Human IRS1 in samples of Adherent Cell Cultures. It is based upon a Cell-Based Phosphorylation assay principle.

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Specifications

Type
 
Cell-Based Phoshorylation ELISA (enzyme-linked immunosorbent assay) kit
Target
 
IRS1
Reactivity
 
Mouse, Human, Rat
Specificity
 
IRS-1 (Phospho-Ser307)
Manual
 

Intended Sample Types
 
Adherent Cell Cultures
Format
 
96-Well Microplate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Qualitative
Storage
 
Short term: 4°C; Long term: see manual.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• User Manual
• 96-well Cell Culture Microplate
• Blocking Buffer
• Detection Antibodies
• HRP-Conjugate
• Substrate
• Stop Solution
• Crystal Violet Solution
• SDS Solution
• Adhesive Plate Sealers

Protocol
 

1. Seed cells into wells and incubate overnight at 37°C, 5% CO2.
2. Apply desired treatment conditions.
3. Add 100 µl of Fixing Solution and incubate 20 minutes at room temperature.
4. Add 100 µl of Quenching Buffer and incubate 20 minutes at room temperature.
5. Add 200 µl of Blocking Buffer and incubate for 1 hour at room temperature.
6. Add 50 µl of 1x primary antibodies and incubate overnight at 4°C. Add 50 µl of HRP-conjugated secondary antibodies and incubate for 1.5 hours at room temperature.
7. Add 50 µl of Ready-to-Use Substrate and incubate for 30 minutes at room temperature.

Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio 100% Guarantee.
About IRS1
 
Uniprot: P35568 NCBI: NM_005544 NP_005535.1

 

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Cell-Based Phosphorylation ELISA Platform Overview