重组细菌外膜蛋白-A

产品英文名称：
Recombinant Bacterial Outer Membrane Protein-A

英文简称:
OmpA

中文名称:
重组细菌外膜蛋白-A

背景资料:
The OmpA protein is one of the main outer-membrane proteins of a large array of Gram-negative bacteria such as A. salmonicida， Shigella dysenteriae and E. coli. OmpA’s major physiological functions include maintenance of the structural integrity and morphology of the cells and porin activity， as well as a role in conjugation and bacteriophage binding. Achromogenic atypical Aeromonas salmonicida is the causative agent of goldfish ulcer disease. Virulence of this bacterium is associated with the production of a paracrystalline outer membrane A-layer protein. The species specific structural gene for the monomeric form of A-protein was cloned into a pET-3d plasmid in order to express and produce a recombinant form of the protein in E. coli BL21（DE3）. The induced protein was isolated from inclusion bodies by a simple solubilization-renaturation procedure and purified by ion exchange chromatography on Q-Sepharose to over 95% pure monomeric protein. Recombinant A-protein was compared by biochemical， immunological and molecular methods with the A-protein isolated from atypical A. salmonicida bacterial cells by the glycine and the membrane extraction methods.

产品描述:
The recombinant form was found to be undistinguishable from the wild type when examined by SDS-PAGE and gel filtration chromatography yielding a 48 kDa monomeric protein. The immunological similarity of the protein samples was demonstrated by employing polyclonal and monoclonal antibodies in ELISA and Western Blot techniques. All forms of A-protein were found to activate the secretion of tumour necrosis factor alpha from murine macrophage. For ref see Maurice et al. （1999） Protein Expression and Purification 16， 396-404. The OmpA is purified by proprietary chromatographic techniques.
来源宿主:
大肠杆菌中重组表达

序列:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Asp-Val-Val-Ile-Ser.

溶解建议:
Sterile Filtered White lyophilized （freeze-dried） powder. It is recommended to reconstitute the lyophilized OmpA in sterile 0.4% NaHCO3.

保存建议：
Lyophilized Bacterial Outer Membrane Protein-A although stable at room temperature for 3 weeks， should be stored desiccated below -18℃. Upon C between 2-7 days and for future usereconstitution OmpA should be stored at 4 below -18℃. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles.
其他简介：

  ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列，专注于蛋白（重组及合成）生产研发，其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际一流的科研级蛋白供应商。目前ProSpec可以提供细胞因子，生长因子，激素，信号蛋白，病毒抗原等近800种重组蛋白和100多种抗体，是世界上提供蛋白品种最多的公司之一，领先的生产工艺和规模使其可以提供毫克到克级蛋白，价格优于同类公司。绝大部分产品是天然成熟型蛋白，而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液，ProSpec-Tany绝大部分产品没有添加保护剂或盐，这就对工艺提出了更高的要求。同时，ProSpec-Tany绝大部分产品为冻干粉，因此易于运输和保存。
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