背景资料: Human IL-17A/F is a 40kDa glycoprotein which is secreted as a disulfide-linked heterodimer. IL-17A/F consists of two proteins of the IL-17 family,IL-17A and IL17F. Proteins of the 6 homodimeric IL17 family show a cysteine knot motif that contains two disulfide-bonds. Human IL17A is produced as a 155 a.a precursor that includes a 23 amino acids signal sequence and a 132 amino acid chain that includes an N-linked glycosylation site. Human IL17F is produced as a 153 amino acid precursor with a 20 amino acid signal sequence and a 133 amino acid region. Similar to IL17A,IL17F also has an N-linked glycosylation site. Both proteins (IL17A & IL17F) share 50% amino acid sequence identity. Human IL17A & IL17F show approximately 60% homology in their amino acid sequence to mouse IL-17A and IL-17F. Interleukin-17A/F and IL17A,IL17F homodimers are manufactured by activted CD4+ T cells,called Th17. IL-23 causes Th17 lymphocytes to manufacture IL-17A/F. IL17RA and IL17RC form a heterodimer for the binding of IL17A and IL17F. IL-17A/F binds IL-17RA. Interleukin-17A/F induces chemokine production and airway neutrophilia with intermediate potency betw小鼠白介素-17A/白介素-17F异二聚体重组蛋白是在大肠杆菌中重组表达的,是由两条非糖基化的多肽链,IL17A单亚基和IL17F单亚基组成的异源二聚体,包含有266个氨基酸,分子量为29.8kDa.该小鼠白介素-17A/白介素-17F异二聚体重组蛋白最终由标准的色谱技术纯化出.n IL17A (most potent) and IL17F (least potent).