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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8度
- 保质期:
1年
- 英文名:
Dengue Polyvalent Gold
- 库存:
999
- 供应商:
上海淳麦
- 规格:
/5ug/20ug/1mg
Recombinant Polyvalent Dengue Antigen, Gold Conjugated
英文简称:
Dengue Polyvalent Gold
中文名称:
重组登革病毒抗原,胶体金标记
背景资料:
Dengue fever is caused by one of four closely related virus serotypes of the genus Flavivirus, family Flaviviridae, patient may have the second dengue fever infected by the di大肠杆菌中重组表达erent serotype virus after the primary infection. Detection of specific antibodies to dengue viruses has bPolyvalent dengue antigen for gold conjugation and rapid test production. This polyvalent dengue antigen contain 3 recombinant dengue envelope proteins, representing all four serotypes, one of them is a fusion protein composed by two di大肠杆菌中重组表达erent serotypes. All the proteins are derived from the envelope. The recombinant proteins for subtype 2 and 3 are 33kDa and 37kDa, respectively, while the size for the fused subtype 1 and 4 envelope protein is about 48kDa in total. Each of these proteins will be supplied individually, individual gold conjugation for each supplied protein and mixture of them together are highly recommended for the rapid test production. The detailed protocol of gold conjugation for each individual protein will be supplied.n used for the diagnosis in clinic. Recently, rapid test has become a most convenient and popular method in the clinical diagnosis. However, there is great di大肠杆菌中重组表达iculty for the manufacturers to have the dengue antigens with the good binding ability to colloid gold, production of strong signal / low background and detection of all four serotype dengue infections for their dengue rapid test development and production.
产品描述:
Polyvalent dengue antigen for gold conjugation and rapid test production. This polyvalent dengue antigen contain 3 recombinant dengue envelope proteins, representing all four serotypes, one of them is a fusion protein composed by two di大肠杆菌中重组表达erent serotypes. All the proteins are derived from the envelope. The recombinant proteins for subtype 2 and 3 are 33kDa and 37kDa, respectively, while the size for the fused subtype 1 and 4 envelope protein is about 48kDa in total. Each of these proteins will be supplied individually, individual gold conjugation for each supplied protein and mixture of them together are highly recommended for the rapid test production. The detailed protocol of gold conjugation for each individual protein will be supplied.
来源宿主:
大肠杆菌中重组表达
序列:
溶解建议:
提供的重组登革病毒抗原,胶体金标记产品为溶液形式.该产品已溶解在含12mM 氯化钠的磷酸盐缓冲液(pH 7.5).
保存建议:
Five years frozen. One month in solution at room temperature.
其他简介:
ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列,专注于蛋白(重组及合成)生产研发,其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际一流的科研级蛋白供应商。目前ProSpec可以提供细胞因子,生长因子,激素,信号蛋白,病毒抗原等近800种重组蛋白和100多种抗体,是世界上提供蛋白品种最多的公司之一,领先的生产工艺和规模使其可以提供毫克到克级蛋白,价格优于同类公司。绝大部分产品是天然成熟型蛋白,而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液,ProSpec-Tany绝大部分产品没有添加保护剂或盐,这就对工艺提出了更高的要求。同时,ProSpec-Tany绝大部分产品为冻干粉,因此易于运输和保存。
上海淳麦生物为ProSpec国内代理销售经销商,详细的产品信息请和我们联系:免费热线:4006815880 微信电话 13770640776
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文献和实验摘 要: 目的:探讨细胞内病毒抗原的超微定位的方法。方法:采用包埋前免疫胶体金方法标记I型单纯疱疹病毒(HSV-1)抗原以研究该病毒在人胚肺细胞(HEL)内的超微定位。结果:发现金颗粒约为5 nm,大小均一,在HSV-1囊膜、内质网膜表面及其邻近区域有大量的金颗粒,而阴性对照在这些地方未见金颗粒。结论:表明包埋前免疫胶体金方法可靠,可用于病毒抗原的超微定位研究。关键词:包埋前免疫胶体金技术;免疫电镜;I型单纯疱疹病毒;病毒抗原中国图书分类号 R392-33 The application
步骤基本与ELISA相同:加样品于固定有配体(抗体或抗原,此处以抗体为例,下同)的硝酸纤维素膜上,通过渗滤在膜中形成抗体-抗原复合物,洗涤渗滤后,再加液体的胶体金标记抗体。当结果为阳性时,在膜上固定有抗体-抗原-胶体金标记抗体复合物而呈现红色斑点。胶体金免疫层析分析CICA原理与GIFA相同,只是操作步骤有所不同,如反应液体流动方向由垂直渗滤改为横向层析流动:样品加样品垫(1)上,样品向吸水垫(4)方向流动,途径载有固相胶体金标记抗体膜(2)后,将金标记物完全复溶,抗原与金标记抗体形成金标记抗体-抗原
immumofiltraition assay GIFA)或滴金免疫分析和胶体金免疫层析分析(gold immunochromatography assay , GICA)。由于GICA更简便快速,已成为目前最主要的快速免疫分析方法之一。 三、原理 胶体金免疫渗滤分析(GIFA)原理与操作步骤基本与ELISA相同:加样品于固定有配体(抗体或抗原,此处以抗体为例,下同)的硝酸纤维素膜上,通过渗滤在膜中形成抗体-抗原复合物,洗涤渗滤后,再加液体的胶体金标记抗体。当结果为阳性时,在膜上固定有抗体-抗原-胶体金标记
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