重组大肠杆菌GroES（HSP10）蛋白

产品英文名称：
Recombinant Human GroEL （HSP60）

英文简称:
GroEL

中文名称:
重组人GroEL（HSP60）蛋白

背景资料:
GroEL， HSP60 is a chaperonin located in the mitochondria which is responsible for the transportation & refolding of proteins from the cytoplasm directly into the mitochondrial matrix. GroEL is regulated by the HSP10 cochaperonin， which is a single heptameric protein ring having a molecular mass of 10 kDa which form a unique complex with HSP60. HSP10， GroES coordinates the ATPase activity of the HSP60 subunits in order to allow the release of bound polypeptide in a manner that is productive for its correct folding. The GroEL protein having the NCBI accession number NP_002147 was purified by using conventional chromatography techniques.

产品描述:
Recombinant GroEL， HSP60 produced in E.Coli is a single， non-glycosylated polypeptide chain containing 593 amino acids and having a molecular mass of 63kDa. The HSP60 is fused to His tag at N-terminus.
来源宿主:
大肠杆菌中重组表达

序列:
MGSSHHHHHH SSGLVPRGSH MLRLPTVFRQ MRPVSRVLAP HLTRAYAKDV KFGADARALMLQGVDLLADA VAVTMGPKGR TVIIEQSWGS PKVTKDGVTV AKSIDLKDKY KNIGAKLVQDVANNTNEEAG DGTTTATVLA RSIAKEGFEK ISKGANPVEI RRGVMLAVDA VIAELKKQSKPVTTPEEIAQ VATISANGDK EIGNIISDAM KKVGRKGVIT VKDGKTLNDE LEIIEGMKFD RGYISPYFIN TSKGQKCEFQ DAYVLLSEKK ISSIQSIVPA LEIANAHRKP LVIIAEDVDG EALSTLVLNR LKVGLQVVAV KAPGFGDNRK NQLKDMAIAT GGAVFGEEGL TLNLEDVQPH DLGKVGEVIV TKDDAMLLKG KGDKAQIEKR IQEIIEQLDV TTSEYEKEKL NERLAKLSDG VAVLKVGGTS DVEVNEKKDR VTDALNATRA AVEEGIVLGG GCALLRCIPA LDSLTPANED QKIGIEIIKR TLKIPAMTIA KNAGVEGSLI VEKIMQSSSE VGYDAMAGDF VNMVEKGIID PTKVVRTALL DAAGVASLLT TAEVVVTEIP KEEKDPGMGA MGGMGGGMGG GMF.

溶解建议:
Sterile filtered colorless solution. The GroEL protein contains 25mM Tris-HCl buffer pH-7.5， 100mM NaCl， 5mM DTT and 10% Glycerol.

保存建议：
Store at 4℃ if entire vial will be used within 2-4 weeks. Store， frozen at -20℃ for longer periods of time. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Avoid multiple freeze-thaw cycles.
其他简介：

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