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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8度
- 保质期:
1年
- 英文名:
Dnak
- 库存:
999
- 供应商:
南京赛泓瑞
- 规格:
/5ug/20ug/1mg
Recombinant Myobacterium Tuberculosis DnaK (HSP70)
英文简称:
Dnak
中文名称:
重组结核杆菌DnaK(HSP70)
背景资料:
DnaK, originally identified for its DNA replication by bacteriophage l in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aregation of stress-denatured proteins.
产品描述:
Recombinant Mycobacterium Tuberculosis Dnak produced in E.Coli is a single, non-glycosylated polypeptide chain containing 625 amino acids and having a molecular mass of 66.7 kDa.
来源宿主:
大肠杆菌中重组表达
序列:
溶解建议:
Sterile Filtered White lyophilized (freeze-dried) powder. The DnaK protein was lyophilized from a concentrated (1mg/ml) solution containing 10mM sodium phosphate buffer pH 7.4, 130mM sodium chloride and 2.5mM KCl. It is recommended to reconstitute the lyophilized DnaK in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
保存建议:
Lyophilized DnaK although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution DnaK should be stored at 4℃ between 2-7 days and for future use below -18℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
其他简介:
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文献和实验Inhibition ofChaperone-Dependent Bacterial Ribosome Biogenesis
In Escherichia coli , the molecular chaperone HSP70 (DnaK) is necessary for 30S and 50S ribosomal subunit assembly at temperatures above 37�C. Inhibitors of DnaK should therefore hinder ribosome biogenesis, in addition
of scaffolding effects and ATP-driven conformational changes that mediate the temporary unfolding and subsequent refolding of protein substrates. DnaK and GroEL are the two major Escherichia coli chaperones. They belong to the HSP70 and HSP60 families of proteins
Stress Detection Using Bioluminescent Reporters of the Heat-Shock Response
. The regulation of the heat shock response typically occurs at the transcriptional level. In Escherichia coli the heat-shock sigma factor, σ32 , drives transcription of about 20 genes (2 ). These include dnaK , encoding the molecular chaperone Hsp70, grpE
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