重组人泛素

产品英文名称：
Recombinant Human U1-snRNP 68kDa

英文简称:
U1snRNP-68

中文名称:
重组人U1-snRNP 68kDa蛋白

背景资料:
Small nuclear ribonucleoprotein complexes （abbreviated as U-snRNP） are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus and consists of one small uridylate-rich RNA （U1 RNA） complexed with several proteins: the thrRecombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein （lacking 66 internal amino acids outside the known epitope-containing areas） fused to a hexahistidine purification tag. The Calculated molecular weight is 44.8 kDa （U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight and the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis）. 68/70 kDa， A， C polypeptides are unique to the U1-snRNP particle， whereas 7 so-called Sm proteins （B/B’， D1， D2， D3， E， F， G） form a core subparticle that is common to all U-snRNP complexes. Both the U1-specific proteins and the Sm core particle are targets of autoantibodies which classically have bRecombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein （lacking 66 internal amino acids outside the known epitope-containing areas） fused to a hexahistidine purification tag. The Calculated molecular weight is 44.8 kDa （U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight and the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis）.n called the RNP and RNP-Sm specificities， respectively. A clean diagnostic distinction of these specificities has bRecombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein （lacking 66 internal amino acids outside the known epitope-containing areas） fused to a hexahistidine purification tag. The Calculated molecular weight is 44.8 kDa （U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight and the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis）.n complicated by the biochemical di大肠杆菌中重组表达iculties of producing clean subparticle fractions from native sources. The use of single recombinant proteins as antigenic targets guarantRecombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein （lacking 66 internal amino acids outside the known epitope-containing areas） fused to a hexahistidine purification tag. The Calculated molecular weight is 44.8 kDa （U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight and the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis）.s a much higher sensitivity and specificity and is the only way to determine RNP antibodies sensu stricto without the disturbing influence of Sm antigens； also with single U1 proteins antibodies will be detected which can be missed because of steric hindrance when using the RNP-Sm complex in an assay. Autoantibodies to U1-snRNP are present in 95% of patients with Mixed Connective Tissue Disease （MCTD） and 30% of patients with SLE. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients. The 68/70 kDa nomenclature of this protein refers to the fact that di大肠杆菌中重组表达erent splice variants of the protein are found in human cells.

产品描述:
Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein （lacking 66 internal amino acids outside the known epitope-containing areas） fused to a hexahistidine purification tag. The Calculated molecular weight is 44.8 kDa （U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight and the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis）.
来源宿主:
大肠杆菌中重组表达

序列:


溶解建议:
Sterile Filtered clear solution. U1-snRNP is supplied in 20mM HEPES buffer pH-7.5， 0.01mM EDTA and 0.02% SDS.

保存建议：
Store at 4℃ if entire vial will be used within 2-4 weeks. Store， frozen at -20℃ for longer periods of time. Avoid multiple freeze-thaw cycles.
其他简介：

  ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列，专注于蛋白（重组及合成）生产研发，其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际一流的科研级蛋白供应商。目前ProSpec可以提供细胞因子，生长因子，激素，信号蛋白，病毒抗原等近800种重组蛋白和100多种抗体，是世界上提供蛋白品种最多的公司之一，领先的生产工艺和规模使其可以提供毫克到克级蛋白，价格优于同类公司。绝大部分产品是天然成熟型蛋白，而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液，ProSpec-Tany绝大部分产品没有添加保护剂或盐，这就对工艺提出了更高的要求。同时，ProSpec-Tany绝大部分产品为冻干粉，因此易于运输和保存。