重组人痉挛性截瘫蛋白21

产品英文名称：
Recombinant Spectrin-alpha

英文简称:
Spectrin alpha

中文名称:
重组人血影蛋白α

背景资料:
Erythrocyte spectrin is the main component of the red cell membrane skeleton responsible for the shape and physical properties of red cells. SPTA1 is a member of the alpha-spectrin gene family. Spectrin is composed of 2 subunits a and b （280 and 246 kDa respectively）. The human erithroid a-spectrin （2418 amino acids） consists of 22 repeating segments of about 106 amino acids in length. SPTA1 forms weaker tetramer interactions than non-erythrocytic alpha spectrin， which may increase the plasma membrane elasticity and deformability of red blood cells. This protein was demonstrated to be a specific substrate for ubiquitination in vitro and in vivo. The main ubiquitination site on human a-spectrin was identified in the lysine 27 of the repeating segment 17 by site directed mutagenesis. Cell-frRecombinant fusion protein: Glutathione-S-Transferase （GST） and a fragment of human a-spectrin （from Lysine 1601 to Leucine 1818） containing repeat 17 and the ubiquitination target lysine 27. The total molecular weight of the fusion protein is 52.4 kDa. experiments using radiolabeled， biotinylated or native ubiquitin and cellular lysates from rabbit retyculocytes， human erythrocytes or k562 cells， showed that only monoubiquitination occurs at this site. Mutations in the SPTA1 gene result in a variety of hereditary red blood cell disorders， such as elliptocytosis type 2， pyropoikilocytosis， and spherocytic hemolytic anemia.

产品描述:
Recombinant fusion protein: Glutathione-S-Transferase （GST） and a fragment of human a-spectrin （from Lysine 1601 to Leucine 1818） containing repeat 17 and the ubiquitination target lysine 27. The total molecular weight of the fusion protein is 52.4 kDa.
来源宿主:
大肠杆菌中重组表达

序列:


溶解建议:
Sterile Filtered clear solution. The protein （1.7mg/ml） contains 50mM Tris buffer pH-8 and 10mM glutathione.

保存建议：
GST-alpha Spectrin although stable at 4℃ for 1 week， should be stored desiccated below -18℃. Please prevent freeze-thaw cycles.
其他简介：

  ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列，专注于蛋白（重组及合成）生产研发，其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际一流的科研级蛋白供应商。目前ProSpec可以提供细胞因子，生长因子，激素，信号蛋白，病毒抗原等近800种重组蛋白和100多种抗体，是世界上提供蛋白品种最多的公司之一，领先的生产工艺和规模使其可以提供毫克到克级蛋白，价格优于同类公司。绝大部分产品是天然成熟型蛋白，而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液，ProSpec-Tany绝大部分产品没有添加保护剂或盐，这就对工艺提出了更高的要求。同时，ProSpec-Tany绝大部分产品为冻干粉，因此易于运输和保存。