重组KISS-1肿瘤转移抑制蛋白

产品英文名称：
Recombinant Human Killer Cell Immunoglobulin-Like Receptor， 3 Domains Long Cytoplasmic Tail 1

英文简称:
KIR3DL1

中文名称:
重组人杀伤细胞免疫球蛋白样受体， 三域 长细胞质尾1

背景资料:
Killer-cell immunoglobulin-like receptors （KIRs）， are a family of cell surface glycoproteins found on Natural Killer （NK） Cells， which are important cells of the immune system. They control the killing function of these cells by interacting with MHC class I molecules， which are expressed on all cell types. This interaction allows them to identify virally infected cells or tumor cells that have a distinctive low level of Class I MHC on their surface. The majority of KIRs are inhibitory， which means that their recognition of MHC suppresses the cytotoxic activity of their NK cell. Only a limited number of KIRs have the capacity to activate cells. The KIR genes are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex （LRC）. KIR molecules are extremely polymorphic， meaning their gene sequences di大肠杆菌中重组表达er significantly betwRecombinant KIR3DL1 produced in E.Coli is a single， non-glycosylated polypeptide chain containing 132 amino acids and having a molecular mass of 15 kDa. The KIR3DL1 is purified by proprietary chromatographic techniques.n individuals， so that di大肠杆菌中重组表达erent individuals have di大肠杆菌中重组表达erent arrays/repertoires of KIR genes. The KIR proteins are categorized by the number of extracellular immunoglobulin domains （2D or 3D） and by whether they have a long （L） or short （S） cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif （ITIM）. Whereas KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The thrRecombinant KIR3DL1 produced in E.Coli is a single， non-glycosylated polypeptide chain containing 132 amino acids and having a molecular mass of 15 kDa. The KIR3DL1 is purified by proprietary chromatographic techniques. Ig-domain from of inhibitory killer cell Ig-like receptor 1（KIR3DL1， NKB1， nkat3， p70KIR） is a NK cell receptor for polymorphic HLA-B determinant. KIR3DLl recognizes the Bw4 determinant defined by sequence motifs at positions 77-83 of the HLA-B heavy chain. The cytoplasmic tail of KIR， which contains two immunoreceptor tyrosine-based inhibition motifs （ITIMs）， mediates inhibitory signal transduction that prevents killer cell-mediated cytotoxicity. A His-tag fusion protein of KIR3DL1 cytoplasmic tail （361-444aa） was overexpressed as insoluble protein aMRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSTSGT IDKLDIEFHLWCSNKKNAAV MDQEPAGNRT ANSEDSDEQD PEEVTYAQLD HCVFTQRKIT RPSQRPKTPP TDTILYTELP NAKPRSKVVS CP.regates （inclusion bodies）.

产品描述:
Recombinant KIR3DL1 produced in E.Coli is a single， non-glycosylated polypeptide chain containing 132 amino acids and having a molecular mass of 15 kDa. The KIR3DL1 is purified by proprietary chromatographic techniques.
来源宿主:
大肠杆菌中重组表达

序列:
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSTSGT IDKLDIEFHLWCSNKKNAAV MDQEPAGNRT ANSEDSDEQD PEEVTYAQLD HCVFTQRKIT RPSQRPKTPP TDTILYTELP NAKPRSKVVS CP.

溶解建议:
Sterile filtered colorless solution. The protein （1mg/ml） contains 25mM Tris-HCl （pH7.5） and 100mM NaCl.

保存建议：
Store at 4℃ if entire vial will be used within 2-4 weeks. Store， frozen at -20℃ for longer periods of time. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Avoid multiple freeze-thaw cycles.
其他简介：

  ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列，专注于蛋白（重组及合成）生产研发，其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际一流的科研级蛋白供应商。目前ProSpec可以提供细胞因子，生长因子，激素，信号蛋白，病毒抗原等近800种重组蛋白和100多种抗体，是世界上提供蛋白品种最多的公司之一，领先的生产工艺和规模使其可以提供毫克到克级蛋白，价格优于同类公司。绝大部分产品是天然成熟型蛋白，而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液，ProSpec-Tany绝大部分产品没有添加保护剂或盐，这就对工艺提出了更高的要求。同时，ProSpec-Tany绝大部分产品为冻干粉，因此易于运输和保存。