重组人杀伤细胞免疫球蛋白样受体， 双域 长细胞质尾1

产品英文名称：
Recombinant Human Interferon Stimulated Gene 15

英文简称:
ISG15

中文名称:
重组人干扰素刺激基因因子15

背景资料:
ISG15 ubiquitin-like Modifier is conjugated to the intracellular target proteins after IFN-alpha or IFN-beta stimulation. ISG15 enzymatic pathway is partially distinct from that of ubiquitin， di大肠杆菌中重组表达ering in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme， but sISG15 Human Recombinant fused to N-terminal Calmudulin Tag （151 a.a.） produced in E.Coli is a single， non-glycosylated polypeptide chain containing a total of 308 amino acids （157 a.a. without tag） and having a molecular mass of 34 kDa.ms to converge with the ub conjugation pathway at the level of a specific E2 enzyme. ISG15 protein targets include STAT1， SERPINA 3G/SPI2A， JAK1， MAPK3/ERK1， PLCG1， EIF2AK2/PKR， MX1/MXA， and RIG-1. Deconjugated by USP18/UBP43 shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. ISG15 serves as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. ISG15 plays a role in autocrine， paracrine and endocrine mechanisms， as in cell-to-cell signaling， possibly partly by inducing ifn-gamma secretion by monocytes and macrophages. ISG15 displays antiviral activity during viral infections. ISG15 in response to IFN-TAU secreted by the conceptus， ligates to and regulate proteins involved in the release of prostaglandin F2-alpha and thus prevent lysis of the corpus luteum and maintain the pregnancy. The interferon-stimulated gene having a molecular mass of 15 kDa is an interferon regulated gene that is induced as a primary response to diverse microbial and cell stress stimuli， and encodes the founding member of the ubiquitin-like protein family. ISG15 shares several common properties with other ubiquitin-like molecules， but its activity is strongly regulated by precise signaling pathways that have a role in innate immunity.

产品描述:
ISG15 Human Recombinant fused to N-terminal Calmudulin Tag （151 a.a.） produced in E.Coli is a single， non-glycosylated polypeptide chain containing a total of 308 amino acids （157 a.a. without tag） and having a molecular mass of 34 kDa.
来源宿主:
大肠杆菌中重组表达

序列:
MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVQMMTAKG SMGWDLTVKM LAGNEFQVSL SSSMSVSELK AQITQKIGVH AFQQRLAVHP SGVALQDRVP LASQGLGPGS TVLLVVDKCD EPLNILVRNN KGRSSTYEVR LTQTVAHLKQ QVSGLEGVQD DLFWLTFEGK PLEDQLPLGE YGLKPLSTVF MNLRLRGG.

溶解建议:
Sterile Filtered colorless solution. The ISG15 solution contains 20mM Tris-HCl pH-8， 100mM NaCl and 1mM DTT.

保存建议：
ISG15 although stable 4℃ for 4 weeks， should be stored desiccated below -18℃. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles.
其他简介：

  ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列，专注于蛋白（重组及合成）生产研发，其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际一流的科研级蛋白供应商。目前ProSpec可以提供细胞因子，生长因子，激素，信号蛋白，病毒抗原等近800种重组蛋白和100多种抗体，是世界上提供蛋白品种最多的公司之一，领先的生产工艺和规模使其可以提供毫克到克级蛋白，价格优于同类公司。绝大部分产品是天然成熟型蛋白，而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液，ProSpec-Tany绝大部分产品没有添加保护剂或盐，这就对工艺提出了更高的要求。同时，ProSpec-Tany绝大部分产品为冻干粉，因此易于运输和保存。