重组人缺氧诱导因子1α，His标签

产品英文名称：
Recombinant Human Hypoxia-Inducible Factor-1 Alpha

英文简称:
HIF1A

中文名称:
重组人缺氧诱导因子1α

背景资料:
Hypoxia-inducible factor-1 （HIF-1）， identified as one of the transcription factors， has bHIF1A Human Recombinant produced in E.Coli is a single， non-glycosylated polypeptide chain containing 298 amino acids （530-826） and having a molecular mass of 32.8 Dalton. The protein migrates as a 40kDa band on SDS-PAGE. The HIF1-A is purified by proprietary chromatographic techniques.n found to play an essential role in cellular and systemic oxygen homeostasis. HIF-1 is a heterodimer composed of HIF-1? subunit and one of thrHIF1A Human Recombinant produced in E.Coli is a single， non-glycosylated polypeptide chain containing 298 amino acids （530-826） and having a molecular mass of 32.8 Dalton. The protein migrates as a 40kDa band on SDS-PAGE. The HIF1-A is purified by proprietary chromatographic techniques. subunits （Hif-1?， Hif-2 （or Hif-3））. The activation of Hif-1 （is closely associated with a variety of tumors and oncogenic pathways. Hif-1 （consists of DNA binding domain （DBD domain）， Dimerization domain and C-terminal regulatory domains， including two transactivation domains （TAD）， an oxygen-dependent degradation （ODD） domain， and inhibitory domains. Under hypoxic conditions HIF1A activates the transcription of more than 40 genes， including， erythropoietin， glucose transporters， glycolytic enzymes， VEGF， and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1A also plays a crucial role in embryonic vascularization， tumor angiogenesis and pathophysiology of ischemic disease. It binds to core DNA sequence 5’-[AG]CGTG-3’ within the hypoxia response element （HRE） of target gene promoters. Activation involves recruitment of transcriptional coactivators such as CREBPB and EP300. Its activity is improved by interaction with both， NCOA1 or NCOA2. Interaction with redox regulatory protein APEX appears to activate CTAD and potentiates activation by NCOA1 and CREBBP. The induction is under reduced oxygen tension. HIF1A is also induced by a variety of receptor-mediated factors such as growth factors， cytokines， and circulatory factors for example PDGF， EGF， FGF-2， IGF-2， TGF-1 beta， HGF， TNF alpha， IL-1 beta， angiotensin-2 and thrombin. Nevertheless， this induction is less intense than that stimulated by hypoxia.

产品描述:
HIF1A Human Recombinant produced in E.Coli is a single， non-glycosylated polypeptide chain containing 298 amino acids （530-826） and having a molecular mass of 32.8 Dalton. The protein migrates as a 40kDa band on SDS-PAGE. The HIF1-A is purified by proprietary chromatographic techniques.
来源宿主:
大肠杆菌中重组表达

序列:
MEFKLELVEK LFAEDTEAKN PFSTQDTDLD LEMLAPYIPM DDDFQLRSFD QLSPLESSSA SPESASPQST VTVFQQTQIQ EPTANATTTT ATTDELKTVT KDRMEDIKIL IASPSPTHIH KETTSATSSP YRDTQSRTAS PNRAGKGVIE QTEKSHPRSP NVLSVALSQR TTVPEEELNP KILALQNAQR KRKMEHDGSL FQAVGIGTLL QQPDDHAATT SLSWKRVKGC KSSEQNGMEQ KTIILIPSDL ACRLLGQSMD ESGLPQLTSY DCEVNAPIQG SRNLLQGEEL LRALDQVN.

溶解建议:
Sterile filtered liquid formulation. The HIF1A recombinant Human solution is formulated 20mM Tris-HCl pH-7.5 and 1mM DTT.

保存建议：
MOP1 Recombinant Human although stable at 4℃ for 30 days， should be stored desiccated below -20℃ for periods greater than 30 days. Please avoid freeze-thaw cycles.
其他简介：

  ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列，专注于蛋白（重组及合成）生产研发，其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际一流的科研级蛋白供应商。目前ProSpec可以提供细胞因子，生长因子，激素，信号蛋白，病毒抗原等近800种重组蛋白和100多种抗体，是世界上提供蛋白品种最多的公司之一，领先的生产工艺和规模使其可以提供毫克到克级蛋白，价格优于同类公司。绝大部分产品是天然成熟型蛋白，而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液，ProSpec-Tany绝大部分产品没有添加保护剂或盐，这就对工艺提出了更高的要求。同时，ProSpec-Tany绝大部分产品为冻干粉，因此易于运输和保存。