重组绿色荧光蛋白

产品英文名称：
Recombinant Human Like Collagen

英文简称:
Collagen

中文名称:
重组类人胶原蛋白

背景资料:
Collagen remained the initial functions of collagen and had some new characters because of its structure， such as reversible colloid with heat and easy processed without molecular reduction and so on. However， animal collagen extracted from animal tissues was insoluble except for the molecular weight reduction. The fetal shortcomings of animal collagen restricted the application is its harmful virus， but Human-like Collagen made up these drawbacks. Collagen （extracted from animal skin， tendons and hides） is potential risk in virus with the potential to cause side e大肠杆菌中重组表达ect and the potential for the contamination of bovine collagen with the fatal mad cow disease. Collagen dissolves in water， which is another advantage to compete with animal collagen. It is di大肠杆菌中重组表达erent from hydrolyzed collagen produced by hydrolyzing animal collagen to short peptide linkage with a lower molecular weight. Human-like collagen was expressed by human collagen gene in host， so mmunogenicity could be lessened much by compared with animal collagen after it entered into human body.

产品描述:
Collagen Human Recombinant produced in E.Coli is a non-glycosylated polypeptide chain a molecular mass of 96，000 Dalton containing human collagen’s cDNA transcribed reversely from mRNA. The Collagen is purified by proprietary chromatographic techniques.
来源宿主:
大肠杆菌中重组表达

序列:
The sequence of the first fifteen N-terminal amino acids was determined and was found to be VLQRRDWENPG. Amino acid %Asp 0.39； Thr 0.16； Ser 5.70； Pro 18.87； Gly 29.17； Ala 13.38； Val .71； Met .18； Ile 0.16； Leu 8.04； Tyr 0.90； Phe 0.15； Lys 0.27； His 0.15； Arg 0.03； Gln 17.40； Hydro Pro 4.34.

溶解建议:
Sterile Filtered White lyophilized （freeze-dried） powder. The protein was lyophilized after from a sterile solution containing no additives. It is recommended to reconstitute the lyophilized Collagen in sterile 18MΩ-cm H2O not less than 100ug/ml， which can then be further diluted to other aqueous solutions.

保存建议：
Lyophilized Collagen although stable at room temperature for 3 weeks， should be stored desiccated below -18℃. Upon reconstitution Collagen should be stored at 4℃ between 2-7 days and for future use below -18℃. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles.
其他简介：

  ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列，专注于蛋白（重组及合成）生产研发，其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际的科研级蛋白供应商。目前ProSpec可以提供细胞因子，生长因子，激素，信号蛋白，病毒抗原等近800种重组蛋白和100多种抗体，是世界上提供蛋白品种最多的公司之一，领先的生产工艺和规模使其可以提供毫克到克级蛋白，价格优于同类公司。绝大部分产品是天然成熟型蛋白，而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液，ProSpec-Tany绝大部分产品没有添加保护剂或盐，这就对工艺提出了更高的要求。同时，ProSpec-Tany绝大部分产品为冻干粉，因此易于运输和保存。