重组人半乳糖凝集素-14

产品英文名称：
Recombinant Mouse Interleukin-27

英文简称:
mIL 27

中文名称:
重组小鼠白介素-27

背景资料:
Interleukin-27 protein is related to IL-12A & is one of the subunits of a heterodimeric cytokine complex. IL-27 interacts with EBV induced gene 3 also called EBI3，and forms a complex that drives rapid expansion of CD4 （+） T cells. IL-27 complex is synergizes with IL-12 in order to triMFP TDPLSLQELR REFTVSLYLA RKLLSEVQGY VHSFAESRLP GVNLDLLPLG YHLPNVSLTF QAWHHSE RLCFLATTLR PFPAMLGGLG TQGTWTSSER EQLWAMRLDL RDLHRHLRFQ VLAAGFKCSK EEEDKEEEEE EEEEEKKLP GALGGPNQVS SQVSWPQLLY TYQLLHSLEL VLSRAVRDLL LLSLPRRPGS AWDS.er the cytokine production of IFN-Gamma of CD4 （+） T cells. The biological e大肠杆菌中重组表达ect of IL-27 is mediated by class-I cytokine receptor （WSX1/TCRR）. The pro-inflammatory activity of IL-27 is mediated through the growing expression of key molecules involved in the MHC class-I & MHC class-II pathways. Both MHC class-I and MHC-class-II expression are increased in endothelial cells after Interleukin-27 stimulation which suMFP TDPLSLQELR REFTVSLYLA RKLLSEVQGY VHSFAESRLP GVNLDLLPLG YHLPNVSLTF QAWHHSE RLCFLATTLR PFPAMLGGLG TQGTWTSSER EQLWAMRLDL RDLHRHLRFQ VLAAGFKCSK EEEDKEEEEE EEEEEKKLP GALGGPNQVS SQVSWPQLLY TYQLLHSLEL VLSRAVRDLL LLSLPRRPGS AWDS.ests that it may play an important role in conferring the immune function on vascular endothelium IL-27p28 subunit can be induced by IFN-beta and during LPS-induced maturation of dendritic cells in type-I IFN-dependent manner through IFN regulatory factor-1 activation. Interleukin-27 regulates Interleukin-12 responsiveness of CD4+ T cells through Stat1-dependent and -independent mechanisms. IL-17 & IL-23 play an important IL-17 role in inflammation. Interleukin-27 possesses potent anti-angiogenic activity that plays an important role in its antitumor and antimetastatic activities. EBV induced gene 3 plays a role，independently from IL-27，in regulating anti-viral or anti-tumoral immune responses. Interleukin-27 is a potent inhibitor of HIV-1 replication in macrophages，CD4+ T cells，peripheral blood mononuclear cells. Interleukin-27 triMFP TDPLSLQELR REFTVSLYLA RKLLSEVQGY VHSFAESRLP GVNLDLLPLG YHLPNVSLTF QAWHHSE RLCFLATTLR PFPAMLGGLG TQGTWTSSER EQLWAMRLDL RDLHRHLRFQ VLAAGFKCSK EEEDKEEEEE EEEEEKKLP GALGGPNQVS SQVSWPQLLY TYQLLHSLEL VLSRAVRDLL LLSLPRRPGS AWDS.ers STAT activation and gene transcription.

产品描述:
小鼠白介素-27重组蛋白是在大肠杆菌中重组表达的，是一条单链、非糖基化的多肽链，包含有207个氨基酸，分子量为23.7kDa.该小鼠白介素-27重组蛋白最终由标准的色谱技术纯化出.
来源宿主:
大肠杆菌中重组表达

序列:
MFP TDPLSLQELR REFTVSLYLA RKLLSEVQGY VHSFAESRLP GVNLDLLPLG YHLPNVSLTF QAWHHSE RLCFLATTLR PFPAMLGGLG TQGTWTSSER EQLWAMRLDL RDLHRHLRFQ VLAAGFKCSK EEEDKEEEEE EEEEEKKLP GALGGPNQVS SQVSWPQLLY TYQLLHSLEL VLSRAVRDLL LLSLPRRPGS AWDS.

溶解建议:
提供的小鼠白介素-27（mIL 27）产品为过滤灭菌的白色冻干粉末；该冻干粉末是从含小鼠白介素-27（mIL 27）的10mM 磷酸钠（NaP）缓冲液（pH 7.5）中冻干产生的.我们建议加无菌的18MΩ-cm水以配制工作储存液到100ug/ml，后继实验过程中可根据实验要求进一步稀释.

保存建议：
小鼠白介素-27（mIL 27）在室温可保存3周，或置于-18℃冻存用于长期保存.重新溶解后可以在4℃冷藏保存至少2-7天；如果想延长保存时间，建议分装后置于-18℃冻存.请避免反复冻融.
其他简介：

  ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列，专注于蛋白（重组及合成）生产研发，其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际的科研级蛋白供应商。目前ProSpec可以提供细胞因子，生长因子，激素，信号蛋白，病毒抗原等近800种重组蛋白和100多种抗体，是世界上提供蛋白品种最多的公司之一，领先的生产工艺和规模使其可以提供毫克到克级蛋白，价格优于同类公司。绝大部分产品是天然成熟型蛋白，而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液，ProSpec-Tany绝大部分产品没有添加保护剂或盐，这就对工艺提出了更高的要求。同时，ProSpec-Tany绝大部分产品为冻干粉，因此易于运输和保存。