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文献和实验Studies of the Ubiquitin Proteasome System
of Ubiquitylated Protein Alternate Protocol 6: Detection of Protein Ubiquitylation Using Tagged Ubiquitin Inhibition of Ubiquitylation and Protein Degradation In Vivo Basic Protocol 12: Use of Dominant Negative Ubiquitin Proteins in Cells Alternate Protocol 7: Use
Production of a His-Tagged Canecystatin in Transgenic Sugarcane
production of transgenic sugarcane coupled with protein purification protocol. In this chapter, we describe production of transgenic sugarcane expressing a His-tagged cystatin under the control of the maize ubiquitin promoter. A transformed sugarcane plant
Ubiquitination of G-Protein-Coupled Receptors
In this chapter we describe methods for detecting the ubiquitination state of G-protein-coupled receptors (GPCRs). This involves coexpression of a GPCR with an epitope-tagged ubiquitin construct in a heterologous expression system. Modification
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