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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8度
- 保质期:
1年
- 英文名:
Protein-A/G
- 库存:
999
- 供应商:
上海淳麦
- 规格:
/5ug/20ug/1mg
Recombinant Protein A/G
英文简称:
Protein-A/G
中文名称:
重组蛋白A/G
背景资料:
Recombinant Protein A/G fusion protein joins IgG binding domains of both Protein A and Protein G. Protein A/G includes four Fc binding domains from Protein A and two from Protein G, yielding a final mass of 50.4 kDa. The binding dependency to pH of Protein A/G lower than Protein A, but has the additive properties of Protein A and G together. Protein A/G binds to all subclasses of human IgG, making it helpful for purifying polyclonal or monoclonal IgG antibodies whose subclasses have not bThe recombinant Protein A/G consists of 7 IgG-binding domains EDABC-C2C3, which corresponds to the Protein A and G domains that are included in the recombinant sequence. The Protein A portion is from Staphylococcus aureus segments E, D, A, B and C. The Protein G portion is from Streptococcus segments C2 and C3. The fusion protein has a predicted molecular mass of 50.5 kDa. But it migrates with an apparent molecular mass of approximately 45kDa in SDS-PAGE.n identifiThe recombinant Protein A/G consists of 7 IgG-binding domains EDABC-C2C3, which corresponds to the Protein A and G domains that are included in the recombinant sequence. The Protein A portion is from Staphylococcus aureus segments E, D, A, B and C. The Protein G portion is from Streptococcus segments C2 and C3. The fusion protein has a predicted molecular mass of 50.5 kDa. But it migrates with an apparent molecular mass of approximately 45kDa in SDS-PAGE.d. Protein A/G binds to IgA, IgE, IgM and IgD. Protein A/G binds to all subclasses of mouse IgG excluding mouse IgA, IgM or serum albumin. This permits Protein A/G to be used in purification and detection of mouse monoclonal IgG antibodies, with no interference from IgA, IgM and serum albumin. Mouse monoclonal antibodies normally have a stronger a大肠杆菌中重组表达inity to the chimeric Protein A/G than to either Protein A or Protein G. Protein A/G also has bThe recombinant Protein A/G consists of 7 IgG-binding domains EDABC-C2C3, which corresponds to the Protein A and G domains that are included in the recombinant sequence. The Protein A portion is from Staphylococcus aureus segments E, D, A, B and C. The Protein G portion is from Streptococcus segments C2 and C3. The fusion protein has a predicted molecular mass of 50.5 kDa. But it migrates with an apparent molecular mass of approximately 45kDa in SDS-PAGE.n used for purification of macaque IgG.
产品描述:
The recombinant Protein A/G consists of 7 IgG-binding domains EDABC-C2C3, which corresponds to the Protein A and G domains that are included in the recombinant sequence. The Protein A portion is from Staphylococcus aureus segments E, D, A, B and C. The Protein G portion is from Streptococcus segments C2 and C3. The fusion protein has a predicted molecular mass of 50.5 kDa. But it migrates with an apparent molecular mass of approximately 45kDa in SDS-PAGE.
来源宿主:
大肠杆菌中重组表达
序列:
溶解建议:
Lyophilized white Powder containing no additives.
保存建议:
2 years at -20℃. After reconstitution, aliquot and store at -20℃. Avoid repeated freeze/thaw cycles.
其他简介:
ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列,专注于蛋白(重组及合成)生产研发,其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际一流的科研级蛋白供应商。目前ProSpec可以提供细胞因子,生长因子,激素,信号蛋白,病毒抗原等近800种重组蛋白和100多种抗体,是世界上提供蛋白品种最多的公司之一,领先的生产工艺和规模使其可以提供毫克到克级蛋白,价格优于同类公司。绝大部分产品是天然成熟型蛋白,而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液,ProSpec-Tany绝大部分产品没有添加保护剂或盐,这就对工艺提出了更高的要求。同时,ProSpec-Tany绝大部分产品为冻干粉,因此易于运输和保存。
上海淳麦生物为ProSpec国内代理销售经销商,详细的产品信息请和我们联系:免费热线:4006815880 微信电话 13770640776
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文献和实验ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
Protein A, derived from the cell wall of Staphylococcus aureus , and protein G, derived from the cell wall of certain strains of b -hemolytic Streptococcci , binds the Fc region
G Protein Abnormalities in Schizophrenia
of a certain receptor subtypes does not always mean that the downstream partners of the receptor (G proteins, effectors, protein kinases, phosphatases) are normal.
Protein G Purification of Antibodies
1. Reagent and Materials(1) Hi-Trap Protein G Column (Pharmacia Biotech #17-0404-01)(2) 20 mM Sodium Phosphate Buffer, pH 7.01.084 g NaH2PO4, anhydrous3.273 g Na2HPO4.7H2Oq.s. to 1 liter with di-H2O(3) 0.1M Glycine, pH 2.83.75 g Glycine1.4 ml HCl
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