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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8°C
- 保质期:
1年
- 英文名:
Long R3 IGF1
- 库存:
999
- 供应商:
南京赛泓瑞
- 规格:
/5ug/20ug/1mg
Recombinant Human Cardiotrophin-Like Cytokine Factor 1
英文简称:
CLCF1
中文名称:
重组人心肌营养素样细胞因子1
背景资料:
Cardiotrophin-like cytokine factor 1 (CLCF1) is a member of the interleukin 6 family of cytokines, which are involved in cell signaling via phosphorylation of gp130. CLCF1 has a sequence of 225 amino acids with a 27 aa signal peptide, having a molecular mass of 22kDa in the mature form, and the maximum homology to cardiotrophin-1 and ciliary neurotrophic factor. CLCF1 is actively secreted from cells by forming a complex with soluble type I CRLF1 or soluble CNTFR. Defects in the CLCF1 gene cause cold-induced sweating syndrome 2 (CISS2). The CISS2 syndrome is typified by profuse sweating after exposure to cold as well as congenital physical abnormalities of the head and spine.
产品描述:
CLCF1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 219 amino acids (28-225 a.a) and having a molecular mass of 24.6kDa. CLCF1 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
来源宿主:
大肠杆菌中重组表达
序列:
MGSSHHHHHH SSGLVPRGSH MLNRTGDPGP GPSIQKTYDL TRYLEHQLRS LAGTYLNYLG PPFNEPDFNP PRLGAETLPR ATVDLEVWRS LNDKLRLTQN YEAYSHLLCY LRGLNRQAAT AELRRSLAHF CTSLQGLLGS IAGVMAALGY PLPQPLPGTE PTWTPGPAHS DFLQKMDDFW LLKELQTWLW RSAKDFNRLK KKMQPPAAAV TLHLGAHGF.
溶解建议:
提供的重组人心肌营养素样细胞因子1产品形式为无菌的澄清溶液.
保存建议:
人心肌营养素样细胞因子1产品在4℃可保存2-4周,或置于-20℃长期保存.推荐添加载体蛋白 (0.1% HSA或BSA)以长期保存,请避免反复冻融.
其他简介:
ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列,专注于蛋白(重组及合成)生产研发,其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际的科研级蛋白供应商。目前ProSpec可以提供细胞因子,生长因子,激素,信号蛋白,病毒抗原等近800种重组蛋白和100多种抗体,是世界上提供蛋白品种最多的公司之一,领先的生产工艺和规模使其可以提供毫克到克级蛋白,价格优于同类公司。绝大部分产品是天然成熟型蛋白,而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液,ProSpec-Tany绝大部分产品没有添加保护剂或盐,这就对工艺提出了更高的要求。同时,ProSpec-Tany绝大部分产品为冻干粉,因此易于运输和保存。
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文献和实验v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
modification(phosphorylation) and this can be measured by immunoblot with specific reagents (indirect), or byimmunoprecipitation and assay (direct), as long as the modification is protected during these procedures.However, inhibition by phosphorylation
uptake positive● Smooth muscle alpha-actin negative推荐的培养基及试剂 ● 内皮细胞生长培养基(2)内皮细胞生长培养基和内皮细胞生长培养基 2 是低血清(2% V/V)培养基,适用于培养大血管内皮细胞。内皮细胞生长培养基 2 缺少内皮细胞生长辅剂(ECGS、牛下丘脑提取物),但含有胰岛素样生长因子(Long R3 IGF)和血管内皮生长因子(VEGF)。一般而言,在培养过程中,VEGF可以使内皮细胞更快速地增殖。最后,由于其对细胞代谢有多方面的影响
Endothelial Cell Systems - Instructions For Use
) 2.0 ml hFGF-B (human Fibroblast Growth Factor - Basic with heparin) 0.5 ml VEGF (Vascular Endothelial Growth Factor) 0.5 ml Ascrobic Acid (Vitamin C) 0.2 ml Hydrocortisone 0.5 ml Long R3-IGF-1 (Human Recombinant Insulin-like Growth Factor) 0.5 ml
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