Hong Kong 1073/99

产品英文名称：
Recombinant Human Protein Tyrosine Phosphatase Non Receptor Type-6

英文简称:
PTPN6

中文名称:
重组人蛋白酪氨酸磷酸酯酶非受体6型

背景资料:
PTPN6 is a member of the protein tyrosine phosphatase （PTP） family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth， di大肠杆菌中重组表达erentiation， mitotic cycle， and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog （SH2） domains， which act as protein phospho-tyrosine binding domains， and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells， and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has bPTPN6 Human Recombinant produced in E.Coli is a single，non-glycosylated polypeptide chain containing 300 amino acids and having a molecular mass of 34.3 kDa. The protein coding region of the catalytic domain of PTPN6 （amino acids 243-541）. The catalytic domain of PTPN6 was overexpressed as insoluble protein aMgfweefes lqkqevknlh qrlegqrpen kgknryknil pfdhsrvilq grdsnipgsdyinanyiknq llgpdenakt yiasqgclea tvndfwqmaw qensrvivmt trevekgrnkcvpywpevgm qraygpysvt ncgehdttey klrtlqvspl dngdlireiw hyqylswpdhgvpsepggvl sfldqinqrq eslphagp人蛋白酪氨酸磷酸酯酶非受体6型（PTPN6）溶液可以在4℃冷藏保存2-4周；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-20℃冻存.请避免反复冻融. vhcsagigrt gt人蛋白酪氨酸磷酸酯酶非受体6型（PTPN6）溶液可以在4℃冷藏保存2-4周；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-20℃冻存.请避免反复冻融.vidmlm enistkgldcdidiqktiqm vraqrsgmvq teaqykfiyv aiaqfiettk kklevlqsqk gqeseygnity.regates （inclusion bodies）. The recombinant PTPN6 protein was purified by FPLC gel-filtration chromatography， after refolding of the isolated inclusion bodies in a redox bu大肠杆菌中重组表达er. Additional amino acid（Met） is attached at N-terminus.n shown to interact with， and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene， which encode distinct isoforms， have bPTPN6 Human Recombinant produced in E.Coli is a single，non-glycosylated polypeptide chain containing 300 amino acids and having a molecular mass of 34.3 kDa. The protein coding region of the catalytic domain of PTPN6 （amino acids 243-541）. The catalytic domain of PTPN6 was overexpressed as insoluble protein aMgfweefes lqkqevknlh qrlegqrpen kgknryknil pfdhsrvilq grdsnipgsdyinanyiknq llgpdenakt yiasqgclea tvndfwqmaw qensrvivmt trevekgrnkcvpywpevgm qraygpysvt ncgehdttey klrtlqvspl dngdlireiw hyqylswpdhgvpsepggvl sfldqinqrq eslphagp人蛋白酪氨酸磷酸酯酶非受体6型（PTPN6）溶液可以在4℃冷藏保存2-4周；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-20℃冻存.请避免反复冻融. vhcsagigrt gt人蛋白酪氨酸磷酸酯酶非受体6型（PTPN6）溶液可以在4℃冷藏保存2-4周；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-20℃冻存.请避免反复冻融.vidmlm enistkgldcdidiqktiqm vraqrsgmvq teaqykfiyv aiaqfiettk kklevlqsqk gqeseygnity.regates （inclusion bodies）. The recombinant PTPN6 protein was purified by FPLC gel-filtration chromatography， after refolding of the isolated inclusion bodies in a redox bu大肠杆菌中重组表达er. Additional amino acid（Met） is attached at N-terminus.n reported.

产品描述:
PTPN6 Human Recombinant produced in E.Coli is a single，non-glycosylated polypeptide chain containing 300 amino acids and having a molecular mass of 34.3 kDa. The protein coding region of the catalytic domain of PTPN6 （amino acids 243-541）. The catalytic domain of PTPN6 was overexpressed as insoluble protein aMgfweefes lqkqevknlh qrlegqrpen kgknryknil pfdhsrvilq grdsnipgsdyinanyiknq llgpdenakt yiasqgclea tvndfwqmaw qensrvivmt trevekgrnkcvpywpevgm qraygpysvt ncgehdttey klrtlqvspl dngdlireiw hyqylswpdhgvpsepggvl sfldqinqrq eslphagp人蛋白酪氨酸磷酸酯酶非受体6型（PTPN6）溶液可以在4℃冷藏保存2-4周；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-20℃冻存.请避免反复冻融. vhcsagigrt gt人蛋白酪氨酸磷酸酯酶非受体6型（PTPN6）溶液可以在4℃冷藏保存2-4周；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-20℃冻存.请避免反复冻融.vidmlm enistkgldcdidiqktiqm vraqrsgmvq teaqykfiyv aiaqfiettk kklevlqsqk gqeseygnity.regates （inclusion bodies）. The recombinant PTPN6 protein was purified by FPLC gel-filtration chromatography， after refolding of the isolated inclusion bodies in a redox bu大肠杆菌中重组表达er. Additional amino acid（Met） is attached at N-terminus.
来源宿主:
大肠杆菌中重组表达

序列:
Mgfweefes lqkqevknlh qrlegqrpen kgknryknil pfdhsrvilq grdsnipgsdyinanyiknq llgpdenakt yiasqgclea tvndfwqmaw qensrvivmt trevekgrnkcvpywpevgm qraygpysvt ncgehdttey klrtlqvspl dngdlireiw hyqylswpdhgvpsepggvl sfldqinqrq eslphagp人蛋白酪氨酸磷酸酯酶非受体6型（PTPN6）溶液可以在4℃冷藏保存2-4周；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-20℃冻存.请避免反复冻融. vhcsagigrt gt人蛋白酪氨酸磷酸酯酶非受体6型（PTPN6）溶液可以在4℃冷藏保存2-4周；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-20℃冻存.请避免反复冻融.vidmlm enistkgldcdidiqktiqm vraqrsgmvq teaqykfiyv aiaqfiettk kklevlqsqk gqeseygnity.

溶解建议:
提供的人蛋白酪氨酸磷酸酯酶非受体6型（PTPN6）产品为过滤灭菌的无色溶液；其中人蛋白酪氨酸磷酸酯酶非受体6型（PTPN6）产品溶解于25mM Tris-HCl，pH 7.5，2mM 巯基乙醇，1mM EDTA，1mMDTT，20%甘油溶液中.

保存建议：
人蛋白酪氨酸磷酸酯酶非受体6型（PTPN6）溶液可以在4℃冷藏保存2-4周；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-20℃冻存.请避免反复冻融.
其他简介：

  ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列，专注于蛋白（重组及合成）生产研发，其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际的科研级蛋白供应商。目前ProSpec可以提供细胞因子，生长因子，激素，信号蛋白，病毒抗原等近800种重组蛋白和100多种抗体，是世界上提供蛋白品种最多的公司之一，领先的生产工艺和规模使其可以提供毫克到克级蛋白，价格优于同类公司。绝大部分产品是天然成熟型蛋白，而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液，ProSpec-Tany绝大部分产品没有添加保护剂或盐，这就对工艺提出了更高的要求。同时，ProSpec-Tany绝大部分产品为冻干粉，因此易于运输和保存。