产品封面图

CRL-2557 Panc 05.04 人胰腺癌细胞

收藏
  • 询价
  • ATCC
  • Panc 05.04
  • 2025年12月15日
    avatar
    上海复祥生物科技有限公司
    14 年金牌会员

  • 企业认证

    点击 QQ 联系

    • 相关产品推荐更多 >

    万千商家帮你免费找货

    0 人在求购买到急需产品
    • 详细信息
    • 询价记录
    • 文献和实验
    • 技术资料
    • 生长状态：

      正常

    • 运输方式：

      常温或干冰

    • 供应商：

      复祥生物

    • 库存：

      大量

    CRL-2557 Panc 05.04 人胰腺癌细胞
    ATCC® Number: CRL-2557™ Price: $417.00
    Designations: Panc 05.04
    Depositors: EM Jaffee
    Biosafety Level: 1
    Shipped: frozen
    Medium & Serum: See Propagation
    Growth Properties: adherent
    Organism: Homo sapiens (human)
    Morphology: epithelial
    Source: Organ: pancreas
    Disease: adenocarcinoma
    Cellular Products: cytokeratins 7 and 18 [50655]
    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    Isolation: Isolation date: May 5, 1995
    Tumorigenic: Yes
    Oncogene: K-ras +
    Antigen Expression: MHC class I +; MHC class II - [50655]
    Blood type B; Rh+
    Age: 77 years adult
    Gender: female
    Ethnicity: White
    Comments: Panc 05.04 is a pancreatic adenocarcinoma epithelial cell line derived, in 1995, from a primary tumor removed from the head-of-the-pancreas of a female with pancreatic adenocarcinoma.
    The cell line exhibits a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. [50655]
    The cells have a reported plating efficiency of 100%. [50655]
    Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 20 Units/ml human recombinant insulin, 85%; fetal bovine serum, 15%
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37.0°C
    Subculturing: Protocol:
    1. Remove and discard culture medium.
    2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
    3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
    4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
    5. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.
    6. Incubate cultures at 37°C.

    Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
    Medium Renewal: Add media once per week. Fluid change one to two times per week.
    Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
    Storage temperature: liquid nitrogen vapor phase
    Doubling Time: 46 hrs
    Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
    recommended serum:ATCC 30-2020
    References: 50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602

    风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。

    • 作者
    • 内容
    • 询问日期
    图标文献和实验
    相关实验

    • 04-流式细胞术上样盲点分析

      流式细胞术上样盲点

    • 04 人体各系统概要：呼吸系统

      叫上鼻甲、中鼻甲和下鼻甲，各鼻甲下方的间隙分别叫上鼻道、中鼻道和下鼻道。上鼻甲的后上方的凹窝叫蝶筛隐窝。各鼻甲与鼻中隔之间的间隙叫总鼻道。切除中鼻甲后，在中鼻道中部可见一个凹向上的弧形裂隙叫半月裂孔，裂孔上方的圆枕形隆起叫筛泡。在中、上鼻道和蝶筛隐窝有鼻旁窦开口，下鼻道有鼻泪管开口。 固有鼻腔粘膜按其性质可分为嗅部和呼吸部。嗅部粘膜覆于上鼻甲以上及其相对的鼻中隔部分，呈淡黄色或苍白色，内含嗅细胞，能感受气味的刺激。其余部分覆以粉红色的呼吸部粘膜，粘膜内含丰富的毛细血管和粘液腺，上皮有纤毛

    • [资源]【整理】蛋白版05年12月无人应助贴

      &id=5004497&sty=1&tpg=21&age=0 [求助]明胶酶谱法检测MMP 想请教一下各位，有谁用明胶酶谱法检测过MMP-9吗？在收集细胞上清液的时候有哪些注意事项，我在文献上看好像上清液还要浓缩，具体怎么做？ http://www.dxy.cn/bbs/post/view?bid=65&id=5006664&sty=1&tpg=21&age=0 [求助]哪家公司的TEV Protease 比较好？ 我们的实验试剂都准备得差不多了，就缺TEV　Protease。10

    查看更多相关实验 >
    图标技术资料

    暂无技术资料 索取技术资料

    同类产品报价

    产品名称
    产品价格
    公司名称
    报价日期
    Panc 05.04细胞, ATCC CRL-2557细胞, Panc0504细胞, 人胰腺癌细胞
    ￥1100
    武汉华尔纳生物科技有限公司
    2025年07月11日询价
    Panc 05.04细胞
    ￥800
    上海联祖生物科技有限公司
    2025年09月08日询价
    Panc05.04
    ￥1320
    广州市左克生物科技发展有限公司
    2025年08月19日询价
    PANC05.04；人胰腺癌细胞
    ¥800
    上海泽叶生物科技有限公司
    2025年07月12日询价
    PANC05.04(人胰腺癌细胞)
    ¥680
    上海钰博生物科技有限公司
    2025年07月15日询价
    CRL-2557 Panc 05.04 人胰腺癌细胞
    询价