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- 详细信息
- 文献和实验
- 技术资料
- 样本:
血清/血浆/组织匀浆液
- 库存:
1000
- 适应物种:
Rat
- 应用:
科研使用
- 检测方法:
ELISA
- 检测范围:
7.813 - 500 pg/ml
- 供应商:
康朗生物
- 规格:
96T
Rat IL9 ELISA Kit (CLIA)
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Product Description
LS-F37624 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat IL9 in samples of Plasma and Serum. It is based upon a Sandwich CLIA assay principle and can be used to detect levels of IL9 as low as 4.688 picograms per milliliter.Specifications
TypeCLIA (chemiluminescent immunoassay) kit
Target
IL9
Reactivity
Rat
Manual
Intended Sample Types
Plasma, Serum
Format
96-Well Strip Plate
Detection
Chemiluminescent
Measurement
Quantitative
Detection Range
7.813 - 500 pg/ml
Sensitivity
4.688
Assay Time
2.5 h
Precision
Intra-Assay: CV<10% Inter-Assay: CV<10%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- User Manual
- 96-well Microplate
- Detection Reagents
- Wash Buffer
- Substrate
- Stop Solution
- Adhesive Plate Sealers
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
About IL9
P15248 NM_000590 NP_000581.1
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Sandwich CLIA Platform Overview
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
should be analyzed. Protein levels can be monitored by Western blot, immunofluorescence, ELISA or other means. (Protein can be isolated using Ambion's PARIS™ Kit.) For quantitating mRNA levels, however, qRT-PCR is again the preferred choice.An example
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