Matrices listed below were spiked with certain level of ATP13A2 and the recovery rates were calculated by comparing the measured value to the expected amount of ATP13A2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-104
95
EDTA plasma(n=5)
85-97
91
heparin plasma(n=5)
90-100
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ATP13A2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.