- $900
- LSBio
- 31.25 - 2000 pg/ml
- LS-F6144
- 美国
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Horse
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
31.25 - 2000 pg/ml
- 供应商：
康朗生物
- 规格：
96T
Horse IL8 / Interleukin 8 ELISA Kit (Sandwich ELISA)
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Product Description
LS-F6144 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Horse IL8 / Interleukin 8 in samples of Cell Culture Supernatants, Cell Lysates, Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of IL8 / Interleukin 8 as low as 13.5 picograms per milliliter.Specifications
TypeSandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
IL8 / Interleukin 8
Reactivity
Horse
Specificity
Interleukin 8 (IL8) No significant cross-reactivity or interference between Interleukin 8 (IL8) and analogs was observed.
Manual
Intended Sample Types
Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
31.25 - 2000 pg/ml
Sensitivity
13.5 pg/ml
Assay Time
4.5 h
Precision
Intra-Assay: CV<10% Inter-Assay: CV<12%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- User Manual
- 96-well Microplate
- Detection Reagents
- Wash Buffer
- Substrate
- Stop Solution
- Adhesive Plate Sealers
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
About IL8 / Interleukin 8
P10145 NM_000584 NP_000575.1
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Sandwich ELISA Platform Overview
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文献和实验ELISA原理与实验方法The advantages of the ELISA are similar to other antibody-labeled reactions which include specificity, sensitivity, inexpensiveness, and safety. Since the enzyme label is the critical portion of ELISA, its selection is very important
chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1, LO/IMEX) 8. reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. B. SOLUTIONS 1. PBS-PF (paraformaldehyde), 2% 2. PBS-tween 20, 0.05% 3. glycine (x
OUTLINE This modification of qualitative ELISA (Enzyme-Linked Immunosorbent Assay) is used for either screening detection of anti-platelet antibodies or for detection of platelet-associated Ig (PAIg) (shown here). PROTOCOL
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