Rat IL21 ELISA Kit (Sandwich E

Rat IL21 ELISA Kit (Sandwich ELISA)

Product Description

LS-F4987 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat IL21 in samples of Cell Culture Supernatants, Cell Lysates, Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of IL21 as low as 6 picograms per milliliter.

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Specifications

Type
 
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
 
IL21
Reactivity
 
Rat
Specificity
 
Interleukin 21 (IL21) No significant cross-reactivity or interference between Interleukin 21 (IL21) and analogs was observed.
Manual
 

Intended Sample Types
 
Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Homogenates
Format
 
96-Well Strip Plate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
15.63 - 1000 pg/ml
Sensitivity
 
6 pg/ml
Assay Time
 
4.5 h
Precision
 
Intra-Assay: CV<10% Inter-Assay: CV<12%
Storage
 
Short term: 4°C; Long term: see manual.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• User Manual
• 96-well Microplate
• Detection Reagents
• Wash Buffer
• Substrate
• Stop Solution
• Adhesive Plate Sealers

Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio 100% Guarantee.
About IL21
 
Uniprot: Q9HBE4 NCBI: NM_021803 NP_068575.1