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- 详细信息
- 文献和实验
- 技术资料
- 形态:
液体
- 保存条件:
4度
- 克隆性:
Polyclonal Antibody
- 标记物:
Purified
- 适应物种:
Human
- 宿主:
Rabbit
- 应用范围:
P*, WB
- 浓度:
请参考说明书
- 靶点:
ENDONUCLEASE G
- 抗体英文名:
RABBIT ANTI ENDONUCLEASE G
- 抗体名:
RABBIT ANTI ENDONUCLEASE G
- 规格:
0.1 mg
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文献和实验Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 0.2 μg/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
23 Endonuclease G Isolation and Assays
Mitochondria in mammalian cells contain a nuclearly encoded, Mg+2 (Mn+2 )-dependent DNA endonuclease activity (reviewed in ref. 1 ), that has been termed Endonuclease G (or Endo G) (2 ). This enzyme activity, an approx 60-kDa homodimer
Partial Endonuclease Digestion
, tubes 2 through 4 contain 20 µl, and tube 5 contains 10 µl (see diagram below). Place the tubes on ice. Add restriction endonuclease (3-10 units of enzyme per µg of DNA) to tube 1, mix quickly, and place the tube on ice. Add 10 µl
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