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- 详细信息
- 文献和实验
- 技术资料
- 形态:
液体
- 保存条件:
4度
- 克隆性:
Polyclonal Antibody
- 标记物:
Purified
- 适应物种:
Mouse
- 宿主:
Goat
- 应用范围:
E, F, IB
- 浓度:
请参考说明书
- 靶点:
IgG IgA IgM
- 抗体英文名:
GOAT ANTI MOUSE IgG/A/M (H/L)
- 抗体名:
GOAT ANTI MOUSE IgG/A/M (H/L)
- 规格:
2 mg
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文献和实验E L I S A w i t h p l a t e l e t s
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
Anti-GFP/BrdU In Situ Immunostaining
Materials 24 well dishes Millicell 0.4 µm inserts (Millipore PICM01250) - coated with fibronectin Culture medium PBS Fixative: 4% paraformaldehyde in PBS (pH 7.6) Rabbit anti-GFP (Molecular Probes, A-11122) FITC-goat anti-rabbit
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
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